Flurbiprofen CAS 5104-49-4 Assay 99.0~100.5% (HPLC) Factory

I-Flurbiprofen
C15H13FO2 244.27
(±) -2- (2-Fluoro-4-biphenylyl) ipropionic acid [5104-49-4].
I-Flurbiprofen iqulethe ngaphantsi kweepesenti ze-99.0 kwaye ingabi ngaphezu kwe-100.5 ipesenti ye-C15H13FO2, ibalwa kwisiseko esomileyo.
Ukupakishwa kunye nokugcinwa- Gcina kwizikhongozeli eziqinileyo.
Imigangatho yezalathiso ye-USP <11>-
USP Flurbiprofen RS
USP Flurbiprofen Related Compound A RS
2-(4-Biphenylyl) propionic acid.
C15H14O2 226.28
Isazisi-
A: I-Infrared Absorption <197K>.
B:Ukufunxwa kweUltraviolet 197U-
Isisombululo: 10 µg ngemL.
Phakathi: 0.1 N sodium hydroxide.
Ubuninzi be-Absorbance kwi-247 nm malunga ne-0.8.
Uluhlu olunyibilikayo <741>: phakathi kwe-114 kunye ne-117.
Ilahleko ekumiseni <731>-Yomise kwi-vacuum kwi-60 ukuya kubunzima obuqhubekayo: ilahlekelwa ngaphezu kwe-0.5% yobunzima bayo.
Intsalela ekutshisweni <281>: hayi ngaphezulu kwe-0.1%.
Iintsimbi ezinzima, Indlela II <231>: 0.001%.
Iikhompawundi ezinxulumene-
I-Diluent-Lungisa umxube wamanzi kunye ne-acetonitrile (11: 9).
Isigaba esiphathwayo-Lungisa umxube ohluziweyo kunye ne-degassed yamanzi, i-acetonitrile, kunye ne-glacial acetic acid (12: 7: 1).Yenza uhlengahlengiso xa kukho imfuneko (jonga ukufaneleka kweNkqubo phantsi kweChromatography <621>).
Isisombululo sesitokhwe esisemgangathweni- Nyibilikisa isixa esinomlinganiso ochanekileyo weUSP Flurbiprofen Related Compound A RS in Diluent ukufumana isisombululo esinoxinzelelo olumalunga nama 50 µg ngemL.
Isisombululo sokufaneleka kwenkqubo-I-Pipet 2.0 mL yesisombululo se-Standard stock kwi-flask ye-volumetric ye-10-mL, yongeza malunga ne-20 mg ye-USP Flurbiprofen RS, ihlambulule nge-Diluent ukuya kwivolumu, kwaye udibanise.
Isisombululo esisemgangathweni-Ukudlulisa i-2.0 mL yesisombululo sesitokhwe esisemgangathweni kwi-flask ye-volumetric ye-10.0-mL, xuba nge-Diluent kwivolumu, kwaye udibanise.
Isisombululo sovavanyo-Lungisa isisombululo se-Flurbiprofen kwi-Diluent equlethe i-2.0 mg nge-mL.
Inkqubo yeChromatographic (jonga i-Chromatography <621>)-I-chromatograph yolwelo ixhotyiswe nge-254-nm detector kunye nekholamu ye-3.9-mm × 15-cm equlethe i-4-µm yokupakisha i-L1.Izinga lokuhamba malunga ne-1 mL ngomzuzu.I-Chromatograph isisombululo sokufaneleka kweSistim, kwaye urekhode iimpendulo eziphezulu njengoko ziqondiswe kwiNkqubo: amaxesha okugcinwa okuhambelanayo malunga ne-0.9 ye-Flurbiprofen ehambelana ne-compound A kunye ne-1.0 ye-flurbiprofen;kunye nokutenxa komgangatho ozalanayo wokuphindaphinda iinaliti akukho ngaphezu kwe-1.0%.
Inkqubo-Ngokwahlukileyo tofa imithamo elinganayo (malunga nama-20 µL) yesisombululo soMgangatho kunye nesisombululo soVavanyo kwichromatograph, urekhode iichromatogram, kwaye ulinganise iindawo zeencopho ezinkulu.Bala ipesenti ye-flurbiprofen enxulumene nekhompawundi A kwinxalenye yeFlurbiprofen ethathwe yifomula:
100(CS / CU)(rU / rS)
apho i-CS lugxininiso, kwi-µg ngemL, ye-USP Flurbiprofen eNxulumene neCompound A RS kwisisombululo esiseMgangathweni;I-CU luxinzelelo, kwi-µg ngemL nganye, yeFlurbiprofen kwisisombululo soVavanyo;kunye ne-rU kunye ne-rS ziyincopho yeempendulo ze-flurbiprofen ehambelana nekhompawundi A efunyenwe kwisisombululo soVavanyo kunye nesisombululo soMgangatho, ngokulandelelana: akukho ngaphezu kwe-0.5% ye-flurbiprofen enxulumene nekhompawundi A ifunyenwe.Bala ipesenti yokungahlambuluki ngakunye kwinxalenye yeFlurbiprofen ethathwe ngefomula:
100(ri / rs)
apho ri yincopho impendulo yokungcola nganye efunyenwe kwisisombululo Test;kunye ne-rs sisimbuku seempendulo zazo zonke iincopho ezifunyenwe kwisisombululo soVavanyo: isixa sabo bonke ukungcola akukho ngaphezu kwe-1.0%.
I-Assay-Dissolve malunga ne-0.5 g ye-Flurbiprofen, ilinganiswe ngokuchanekileyo, kwi-100 mL yotywala, ngaphambili ingathathi hlangothi nge-0.1 N sodium hydroxide VS kwi-phenolphthalein endpoint, yongeza i-phenolphthalein TS, kunye ne-titrate nge-0.1 Nsodium hydroxide VS kwimbonakalo yokuqala ye-faint ye-faint yokuqala. umbala opinki ozingisayo ungekho ngaphantsi kwemizuzwana engama-30.I-mL nganye ye-0.1 N i-sodium hydroxide ilingana ne-24.43 mg ye-C15H13FO2.