H-Glu(OEt)-OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity >99.0% (HPLC) Factory

I. Iinkqubo zokuhlolwa
1. Indawo yokunyibilika
Kusetyenziswa isixhobo sedijithali sokunyibilika
Inyathelo 2: Umxholo
2.1 IiReagents
Inyathelo loku-1: I-asidi yefom
2. I-acetic acid ye-glacial
3. I-Perchloric acid isisombululo somgangatho wetitration (C=0.1000mol/l)
4. I-Crystal Violet isalathisi solwelo: 5g / l.
2.2 Izixhobo kunye nezixhobo
1. Ibeaker 150ml
2. Microburet: 10ml
2. 3 Uhlalutyo lwamanyathelo
2. 3. Ndiyilinganisa ngokuchanekileyo malunga ne-300mg yesampuli eyomisiwe ngaphambili kwi-105 ℃ ye-3h, ndiyifake kwi-bethel ye-150ml, yongeza i-3ml ye-formic acid, yongeza i-50ml ye-ice acetic acid, yongeza i-2 drops ye-crystal violet test solution ( TS-74), kunye ne-titrate kunye ne-0.1mol / L ye-perchloric acid kude kube kwindawo yesiphelo esiluhlaza okanye ide i-blue iphele ngokupheleleyo.
2. 3. 2 Indlela yokubala
X ilingana no C (V1-V2) amaxesha 0.2042 / M amaxesha 100
C luxinaniso lwesisombululo se-titration esisezantsi se-perchloric acid, esichazwe kwi-moles nganye ilitha (mol / l).
I-V1 ngumthamo osetyenzisiweyo we-perchloric acid esemgangathweni we-titration isisombululo esigxininiswe kwisampulu ye-titration, echazwe kwi-ml (ml).
Umthamo osetyenzisiweyo wesisombululo setitration esiqhelekileyo se-perchloric acid kuvavanyo olungenanto lwe-V2, oluchazwe nge-ml (ml)
I-0.2042 kunye ne-1.00ml ye-perchloric acid esemgangathweni yesigaba solwelo esigxininisiweyo xa umgangatho wale mveliso ubonakaliswa kwi-G
M Umgangatho wesampulu, echazwe ngeegram (g).
2. 4 Ukulungiswa
Ukuba umahluko wobushushu begumbi phakathi kwesisombululo sovavanyo lwe-titration kunye nesisombululo somgangatho we-perchloric acid yokulinganisa ungaphezulu kwe-10℃, kufuneka silinganiswe kwakhona;Ngaphandle koko, i-concentration C1 yesisombululo esiqhelekileyo se-perchloric acid iya kulungiswa ngokovavanyo (2).
C2= C1/1+0.0011 (t1-t2)
I-1+0.0011 yi-coefficient yokwandisa i-acetic acid ye-glacial
Iyunithi yobushushu begumbi lokulinganisa i-T2 ye-perchloric acid yiCelsius (° C).
Iyunithi yobushushu begumbi apho isampuli ifakwe titrated nguCelsius (° C).
I-C1 t2℃ i-perchloric acid esemgangathweni yoxinzelelo lwesisombululo, kwi-moles ilitha nganye (MOL/L)
3. Yomile kwaye ayinabunzima
3.1 Izixhobo:
I-oven yokumisa i-Thermostatic, ibhalansi ye-1/10,000.
3.2 Inkqubo:
Thatha i-1-2 grams yeesampulu (echanekile ukuya kwi-0.5mg) kwaye uyibeke kwi-pre-yomisiweyo yobunzima obuqinileyo kunye nobunzima bebhotile.Iisampuli ziya kuba nobukhulu obufanayo phantsi kwebhotile yokulinganisa.Ngokuphathelele iisampuli, ziya komiswa kwi-oven ye-thermoelectric (105 ± 5℃) ukuya kubunzima obuqhubekayo.
3.3 Ukubala
U-X ngu-m thabatha m1 wahlulwe ngo-m thabatha m2 umphinda-phinde ngo-100
Apho: X- isiqulatho sokufuma %
m1 -- Ubunzima besampulu eyomileyo kunye nebhotile yokulinganisa g
m2 - Ukulinganisa ubunzima bebhotile g
m- Ubunzima besampulu g
3.4 Iingongoma zokuqwalaselwa
1. Amanqaku okuqwalaselwa: 1. Iisampuli ezihambelanayo kufuneka zenziwe ukuze zomiswe kwaye zingabi nasisindo.Isampuli enye ineziphumo ezimbini, kwaye impazamo phakathi kweziphumo ezimbini ayikho ngaphezu kwe-0.5%.
2. Ukulinganisa kufuneka kulumke, kuchaneke ukuya ku-0.0001.
4 ukuya kujikelezo
4.1 Imigaqo-siseko
Ukukhanya kwe-Plane-polarized kudlula kulwelo okanye isisombululo sekhompawundi equlethe umsebenzi othile we-optical (oko kukuthi, i-optical active substance) inokubangela ukujikeleza kwe-optical, okubangela ukuba i-plane yokukhanya kwe-polarized ijikeleze ngakwesobunxele okanye ngasekunene.Inani leedigri zokujikeleza libizwa ngokuba yi-optical rotation.Kwiqondo lobushushu elithile (elidla ngokubonakaliswa ngu-t, linokuba yi-20℃ okanye i-25℃) ubude obuthile bokukhanya (ukukhanya kwesodium etyheli kunye no-D, ubude be-wavelength λ 589.3nm) ukukhanya okucandisiweyo ngemililitha nganye equlethe i-1g yemathiriyeli yokubona, ubukhulu be-1dm (oko kukuthi, i-10cm) isisombululo xa ujikelezo lubizwa ngokuba lujikelezo oluthile lwe-optical.
4.2 Iimfuno
Umgca we-D we-sodium spectrum (589. 3 nm) yayisetyenziselwa ukulinganisa ubude betyhubhu ye-1dm kunye nobushushu be-20 ℃.
4.3 Izixhobo zokusebenza
4.3.1 I-autotropy yedijithali (ukuchaneka ±0.001 °)
4.3.2 Ibhalansi ye-elektroniki (ukuchaneka 0.0001g).
4.4 Indlela yokuSebenza
Thatha i-3g yale mveliso, echanekileyo kwi-0.0001g, uyibeke kwibhotile ye-volumetric ye-100ml, yongeza i-ethanol ukuze uchithe kwaye uyihlambulule kwisikali, xubha kakuhle.Hlanza ityhubhu yokulinganisa ngexabiso elincinane lolwelo amatyeli aliqela, uze ngokucotha ugalele ulwelo kwityhubhu yokulinganisa (musa ukwenza amaqamza enzeka), beka ityhubhu yokulinganisa kwigyroscope ukuze ibonwe, kwaye ufunde ukufundwa kokujikeleziswa kwamehlo Amaxesha ama-3 ngokuhambelana.
4.5 Ukubala
Ukujikeleza okuthe ngqo kwe-L-glutamate Diethyl carbonate kwi-sodium spectrum D-line kwi-20℃ [Alpha]D20 ibalwe ngolu hlobo lulandelayo:
[ɑ] D20 = alpha/m
Apho: α -- iavareji yenani le-cubic rotation readings;
m -- umgangatho wesampuli.
4.6 Umahluko ovumelekileyo: Umahluko opheleleyo wesampulu efanayo awuyi kudlula i-0.02% xa ulinganiswe kabini.
4.7 Izilumkiso
4.7.1 Isinyibilikisi kufuneka sisetyenziswe njengolungelelwaniso olungenanto phambi komlinganiselo ngamnye: Emva komlinganiselo, ulungelelwaniso kufuneka lwenziwe kwakhona ukuqinisekisa ukuba inqaku elinguziro liyatshintsha na ngexesha lomlinganiselo.Ukuba kukho naluphi na utshintsho, ukujikeleza kwe-optical yesampuli kufuneka kumiselwe kwakhona.
4.7.2 Isampuli kufuneka icaciswe emva kokuchithwa kunye nokuhlanjululwa;kungenjalo, kufuneka ihluzwe.
Inyathelo lesi-5 Imbonakalo
Imveliso iya kuba Mhlophe ukuya kumhlophe-mhlophe microcrystalline powder