L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Uvavanyo 98.5~101.0% (Titration) Umgangatho ophezulu weFactory

I-L-Cysteine ​​iqulethe ngaphantsi kwe-98.5% kwaye ingabi ngaphezu kwe-101.0% ye-L-Cysteine ​​​​(C3H7NO2S), ibalwa kwisiseko esomileyo.
Inkcazo L-Cysteine ​​yenzeka njengeekristale ezimhlophe okanye umgubo omhlophe wekristale.Inevumba elibonakalayo kunye nencasa ehlabayo.Inyibilika ngokukhululekileyo emanzini, kwaye ayinyibiliki kwi-ethanol (99.5).Inyibilika kwi-1 mol / L i-hydrochloric acid TS.
Ukuchonga Ukumisela i-spectrum ye-infrared yokufunxa ye-L-Cysteine ​​njengoko iqondiswe kwindlela yedisk ye-potassium bromide phantsi kwe-Infrared Spectrophotometry <2.25>, kwaye uthelekise i-spectrum kunye ne-Reference Spectrum: zombini i-spectra ibonisa ukuqina okufanayo kwi-absorption kumanani amaza afanayo.
Ukujikeleza kwe-Optical <2.49> [a] 20D: + 8.0 ~ + 10.0 ° (2 g ibalwa kwisiseko esomileyo, i-1 mol / L i-hydrochloric acid TS, 25mL, 100 mm).
pH <2.54> I-pH yesisombululo esilungiselelwe ngokuchithwa kwe-1.25 g ye-L-Cysteine ​​​​kwi-50 mL yamanzi ngu-4.7 ukuya ku-5.7.
Ukucoceka (1) Ukucaca kunye nombala wesisombululo-Dibanisa i-1.0 g ye-L-Cysteine ​​kwi-20 mL yamanzi: isisombululo sicacile kwaye singenambala.
(2) I-Chloride <1.03>-Dibanisa i-0.30 g ye-L-Cysteine ​​kwi-10mL ye-nitric acid ehlanjululweyo (1 ku-4), yongeza i-10mL ye-hydrogenperoxide (30), ukushisa imizuzu engama-20 kwibhafu yamanzi abilayo, epholile, uze wongeze amanzi ukwenza 50 mL.Yenza uvavanyo usebenzisa esi sisombululo njengesisombululo sovavanyo.Lungisa isisombululo sokulawula kunye ne-0.35 mL ye-0.01 mol / L i-hydrochloric acid VS (kungekho ngaphezu kwe-0.041%)
(3) I-Sulfate <1.14> -Dibanisa i-0.6 g ye-L-Cysteine ​​kwi-30 mL yamanzi kunye ne-3 mL ye-dilute hydrochloric acid, kwaye yongeza amanzi ukwenza i-50 mL.Yenza uvavanyo usebenzisa esi sisombululo njengesisombululo sovavanyo.Lungiselela isisombululo solawulo ngolu hlobo lulandelayo: Ukuya kwi-0.35 mL ye-0.005 mol / L sulfuric acid VS yongeza i-3 mL ye-dilute hydrochloric acid kunye namanzi ukwenza i-50 mL.Lungiselela isisombululo sovavanyo kunye nesisombululo sokulawula kunye ne-4 mL ye-barium chloride TS, ngokulandelanayo (kungekho ngaphezu kwe-0.028%).
(4) Ammonium <1.02>-Yenza uvavanyo nge-0.25 g ye-L-Cysteine, usebenzisa i-distillation phantsi koxinzelelo oluncitshisiweyo.Lungiselela isisombululo sokulawula kunye ne-5.0 mL ye-Standard Ammonium Solution (engekho ngaphezu kwe-0.02%).
(5) I-Heavy Metals <1.07>-Qhubeka kunye ne-1.0g ye-L-Cysteine ​​​​ngokweNdlela ye-4, kwaye wenze uvavanyo.Lungisa isisombululo sokulawula kunye ne-1.0 mL ye-Standard Lead Solution (engekho ngaphezu kwe-10 ppm).
(6) Intsimbi <1.10> -Lungisa isisombululo sovavanyo kunye ne-1.0 g ye-L-Cysteine ​​ngokweNdlela ye-1, kwaye wenze uvavanyo ngokweNdlela A. Lungisa isisombululo sokulawula kunye ne-1.0mL ye-Standard Iron Solution (kungekho ngaphezu kwe-10 ppm ).
(7) Izinto eziNxulumeneyo-Dibanisa i-0.10 g ye-L-Cysteine ​​kwisisombululo se-N-ethylmaleimide (1 kwi-50) ukwenza ngokuthe ngqo i-10 mL, shiya imizuzu engama-30, kwaye usebenzise esi sisombululo njengesisombululo sesampuli.Umbhobho we-1 mL wesisombululo sesampuli, yongeza amanzi ukwenza ngokuthe ngqo i-10 mL, ipayipi ye-1 mL yesi sisombululo, yongeza amanzi ukwenza i-50 mL ngqo, kwaye usebenzise esi sisombululo njengesisombululo esiqhelekileyo (1).Ngokwahlukileyo, chitha i-0.10 g ye-L-cystine kwi-0.5mol / L i-hydrochloric acid TS ukwenza i-20 mL ngqo.Umbhobho we-1mL yesi sisombululo, yongeza amanzi ukwenza i-100 mL, kwaye usebenzise esi sisombululo njengesisombululo esiqhelekileyo (2).Yenza uvavanyo ngezi zisombululo njengoko kuqondiswe phantsi kwe-Thin-layer Chromatography <2.03>.I-Spot 10mL nganye yesisombululo sesampuli kunye nezisombululo eziqhelekileyo (1) kunye (2) kwi-plate ye-silica gel ye-chromatography encinci.Phuhlisa ipleyiti ngomxube we-1-butanol, amanzi, kunye ne-acetic acid (100) (3: 1: 1) ukuya kumgama omalunga ne-10 cm, kwaye womise ipleyiti imizuzu engama-30 kwi-809C. Tshaza ipleyiti ngokulinganayo ngesisombululo. ye-ninhydrin kumxube we-methanol kunye ne-acetic acid (100) (97: 3) (1 kwi-100), kwaye emva koko ishushu kwi-80 ℃ imizuzu eyi-10: indawo efunyenwe kwisisombululo sesampuli ehambelana nendawo efunyenwe kwisisombululo esiqhelekileyo. (2) alinamandla ngaphezu kwendawo ukusuka kwisisombululo esisemgangathweni (2).Kwakhona, amabala angaphandle kwendawo eyintloko kunye namabala akhankanywe ngasentla kwisisombululo sesampulu awaqinanga ngaphezu kwendawo evela kwisisombululo esiqhelekileyo (1).
Ilahleko ekomileyo <2.41> Akukho ngaphezu kwe-0.50% (1 g, kwi-vacuum, i-phosphorus (V) i-oxide, iiyure ze-3).
Intsalela kwi-Ignition <2.44> Akukho ngaphezu kwe-0.1% (1 g).
Uvavanyo Yenza umlinganiselo ngokuchanekileyo malunga ne-0.2 g ye-L-Cysteine, beka i-itin kwi-flask evaliweyo, kwaye unyibilike kwi-20 mL yamanzi.Dissolve i-4 g ye-iodide ye-potassium kwesi sisombululo, ngokukhawuleza ubeke emanzini abandayo e-ice, yongeza i-5 mL ye-dilute hydrochloric acid kunye ne-25 mL ngqo ye-0.05 mol / L ye-iodine VS, shiya kwindawo emnyama imizuzu engama-20, kwaye emva koko i-titrate<2.50 >isixa esigqithisileyo se-iodine ene-0.1 mol/L yesodium thiosulfate VS (isalathisi: istatshi TS).Yenza ukuzimisela okungenanto usebenzisa indlela efanayo.
I-mL nganye ye-0.05 mol/L iodine VS＝12.12 mg ye-C3H7NO2S
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