L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Uvavanyo >99.0% Factory

1. Imbonakalo
Thatha umlinganiselo ofanelekileyo wale mveliso, uyibeke kwiphepha elimhlophe eligudileyo kwaye usasaze u-5 cm2 kunye no-5 mm ubukhulu.Yijonge kwindawo eqaqambileyo.
2. Ubunyulu (HPLC)
2.1 Isixhobo
Ukusebenza okuphezulu kwechromatography yolwelo, umtshina wePDA.
Ibhalansi yohlalutyo lwe-elektroniki
2.2 IiReagents
I-Acetonitrile (ibakala lechromatographic), TFA(ibakala lechromatographic)
2.3 Iimeko zeChromatographic
2.3.1 Umhlathi: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Ukubona ubude bobude: INC220nm
Izinga lokuhamba: 1.0mL / min
Umthamo wokutofa:10 μL (ukwenzela isalathiso)
Diluent: Acetonitrile
Ixesha lokubaleka: imizuzu engama-20
2.4 Ukulungiswa kwesigaba esiphathwayo
Isigaba se-Mobile A (i-0.1% yamanzi e-trifluoroacetic acid): ukufunxa ngokuchanekileyo i-2.0ml TFA, Nciphisa kwi-2000ml ngamanzi, xuba kunye ne-degas;
Isigaba se-Mobile B (0.1% trifluoroacetic acid acetonitrile): ukufunxa ngokuchanekileyo i-2.0ml TFA, Nciphisa kwi-2000ml nge-acetonitrile, ukuxuba kunye ne-degas;
2.5 Iiparamitha zesixhobo
Ixesha (umzu) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Ukulungiswa kwesisombululo sesampuli
Ubunzima kwaye uchithe isampuli ye-0.1g kunye ne-acetonitrile, i-dilute ukuya kwi-100ml, xubha kakuhle ukusetyenziswa, okanye ukugxila okufanayo.Lungiselela iisampuli ezimbini ngokuhambelanayo.
2.7 Ukumiselwa kweSampuli
Hlalutya isampuli ngokwenkqubo yesampulu ilandelayo:
Ngaphezulu kwesitofu esinye sesisombululo esingenanto
1 isampulu yenaliti isisombululo 1#
1 isampuli yenaliti isisombululo 2#
2.8 Ubalo lweziphumo
2.8.1 Indlela yokulinganisa indawo eyincopho yasetyenziselwa ukubala ukucoceka kweHPLC ngokutsala indawo engenanto.
2.8.2 Ukutenxa kuthetha ukutenxa kococeko lweenaliti ezimbini aziyi kuba nkulu kune-1%
2.8.3 Ukuba iziphumo zazo zombini iinaliti ziyahlangabezana nemilinganiselo yokwamkelwa, ucoceko oluqhelekileyo luthathwa njengesiphumo sokugqibela.
3, Indawo yokunyibilika -- RY-1 isixhobo sendawo yokunyibilika
4. Ilahleko kwindlela yokuvavanya ukomisa
4.1 Izixhobo:
I-oven yokumisa i-thermostatic yombane, ibhalansi ye-1/10,000.
4.2 Inkqubo:
Kwibhotile yokulinganisa i-flat kunye nesisindo esingaguqukiyo kunye nesigqubuthelo somlomo womhlaba owomileyo, linganisa i-gram ye-1 (echanekileyo ukuya kwi-0.0001 gram) yesampuli.Isampulu kufuneka isasazwe ngokulinganayo emazantsi ebhotile yokulinganisa ngobunzima obungekho ngaphezu kwe-10mm, ifakwe kwi-oven yokumisa yombane ye-thermostatic, yome kwi-105 ~ 110 ℃ ngeeyure ze-3, kwaye emva koko ungene kwigumbi lokumisa ukuze uphole. ubushushu begumbi lokulinganisa.
Ukubala: Ilahleko ekomisweni %= (M1-M2) ÷M×100
Kuphi: M1: ubunzima besampulu kunye nebhotile yokulinganisa ngaphambi kokumisa, iigram
I-M2: ubunzima besampuli kunye nebhotile yokulinganisa emva kokumisa, kwiigram
M: Ubunzima besampulu, iigram
5. Ukujikeleza okuthe ngqo
-- Ukujikeleza okuthe ngqo kulinganiswa ngokuhambelana ne-GB/T613-1988
Ukulungiswa kwesampuli: isampuli ye-1.0000g yalinganiswa ngokuchanekileyo kwaye yahanjiswa kwibhotile ye-volumetric ecocekileyo neyomileyo ye-50ml, amanzi angama-20ml yongezwa, ibhotile ifakwe i-capped kwaye igubungele ukuchithwa, kwaye ihlanjululwe kwisikali ngamanzi.
Uvavanyo: Lungisa i-zero ye-gyroscope ngaphambi kovavanyo, uze ulayishe ityhubhu yokuvavanya kunye nesisombululo sesampuli, urekhode i-Angle yokujikeleza, kwaye ubale ukujikeleza okuthe ngqo kwesampuli kunye nefomula elandelayo.
[а]D20 = (r×50) ÷ (L×W)
[а]D20: Ukujikeleza okuthe ngqo kwi-20 ℃ yesisombululo sesampuli
r: Ukujikeleza okubonakalayo kwi-20 ℃ kwisisombululo sesampuli
50: Umthamo wesisombululo sesampulu esilungisiweyo (ml)
w: Ubunzima besampulu (g)
L: Ubude betyhubhu ejikelezayo yokubona (dm)