L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Uvavanyo 98.5 ~ 101.5% Umgangatho ophezulu weFactory

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Indlela yoVavanyo
Ukuchonga: Thelekisa i-spectrum ye-infrared yokufunxa yesampuli kunye neyomgangatho we-potassium bromide disc method.
Ukujikeleziswa okuthe ngqo [α]20/D: Isampulu eyomileyo, C=5, 1mol/L HCl
I-State of Solution (i-Transmittance): i-0.5g kwi-10ml ye-1mol / L HCl, i-spectrophotometer, i-430nm, i-10mm ubukhulu beseli.
I-Chloride (Cl): 0.7g, A-1, ref: 0.40ml ye-0.01mol/L HCl
I-Ammonium (NH4): B-2
I-Sulfate (SO4): 1.2g, (1), i-ref: 0.50ml ye-0.005mol/L H2SO4
Intsimbi (Fe): 1.5g, (2), ref: 1.5ml ye-Iron Std.(0.01mg/ml)
Iintsimbi ezinzima (Pb): 2.0g, (2), ref: 2.0ml yePb Std.(0.01mg/ml)
Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ye-As2O3 Std.
Ezinye iiAmino Acids: Dilute ammonia R2 Test Sample: 50μg, F-6-a, control: L-Tyr 0.25μg
Ilahleko ekomisweni: kwi-105℃ kwiiyure ezi-3.
Intsalela kwi-Ignition (Sulfated): Uvavanyo lwe-AJI 13
Uvavanyo: Isampulu eyomileyo, i-180mg, (1), i-6ml ye-asidi ye-formic, i-50ml ye-glacial acetic acid, i-0.1mol / L HCLO4 1ml = 18.119mg C9H11NO3
Uvavanyo lwe-pH: 0.02g kwi-100ml egcwele isisombululo samanzi

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) USP35 Indlela yoVavanyo
INGCACISO
I-Tyrosine iqulethe i-NLT 98.5% kunye ne-NMT 101.5% ye-L-Tyrosine (C9H11NO3), ibalwe ngokomisiweyo.
UKUCHAZA
A. UKUFUTSHWA KWE-IFRARED <197K>
UVAVANYO
INKQUBO
Isampulu: 180 mg yeTyrosine
Akunanto: Xuba i-6 mL ye-asidi ye-formic kunye ne-50 mL ye-glacial acetic acid.
Inkqubo yeTitrimetric
(Jonga i-Titrimetry <541>)
Imowudi: Ukubhalwa ngokuthe ngqo
I-Titrant: 0.1 N i-perchloric acid VS
Ukubonwa kwendawo yokugqibela: I-Potentiometric
Uhlalutyo: Chitha iSampulu kwi-6 mL ye-asidi ye-formic kunye ne-50mL ye-glacial acetic acid, kunye ne-titrate kunye ne-Titrant.Yenza ukuzimisela kweGank.
Bala ipesenti yetyrosine (C9H11NO3) kwiSampuli ethathiweyo:
Isiphumo = {[(VS-VB) x N x F]/W} x100
VS= umthamo weTitrant esetyenziswe yiSampuli (mL)
VB= umthamo we-Titrant esetyenziswe yi-Gank (mL)
N= eyona nto iqhelekileyo ye-Titrant (mEq/mL)
F= i-equivalency factor, 181.2 mg/mEq
W= Ubunzima besampulu (mg)
Iinqobo zokwamkela: 98.5% ~ 101.5% kwisiseko esomileyo
UBUNGCOLA
OKUSHIYELWE OKUSHIYELWE <281>: NMT 0.4%
I-CHLORIDE NE-SULFATE, i-Chloride <221>
Isisombululo esisemgangathweni: 0.20mL ye-0.020 N i-hydrochloric acid
Isampulu: 0.35 g yeTyrosine.[QAPHELA-Xa kukho imfuneko, nyibilikisa iSampuli ngokuyifudumeza isondele ebilayo kwaye wongeze i-1 mL ye-nitric acid.]
Indlela yokwamkelwa: NMT 0.04%
I-CHLORIDE NE-SULFATE, i-Sulfate <221>
Isisombululo esisemgangathweni: 0.50mL ye-0.020 N sulfuric acid
Isampulu: 1.2 g yeTyrosine.[QAPHELA-Ukuba kukho imfuneko, nyibilikisa iSampuli ngokudibanisa i-6 mL ye-diluted hydrochloric acid.]
Indlela yokwamkelwa: NMT 0.04%
I-IRON <241>: NMT 30ppm [QAPHELA-Ukuba kuyimfuneko, sebenzisa i-2 mL ye-hydrochloric acid ukunyibilikisa iSampule.]
IIMETALI EZINZIMA, Indlela I <231>: NMT 15ppm
EZINXULUMENEYO
Isisombululo se-ammonia edibeneyo: Nciphisa i-16 mL ye-ammonia egxininisiweyo ngamanzi ukuya kwi-100 mL.
Isisombululo sokufaneleka kwenkqubo: Dissolve 10 mg ye-USP L-Tyrosine RS kunye ne-10 mg ye-USP L-Phenylalanine RS kwi-1 mL ye-Diluted ammonia isisombululo, kwaye uhlambulule ngamanzi kwi-25.0 mL.
Isisombululo esisemgangathweni: Kwi-flask efanelekileyo, chitha ubuninzi be-USP L-Tyrosine RS kwi-1 mL ye-Diluted ammonia solution.Nciphisa ngamanzi ukuya kumthamo ukuze ufumane isisombululo se-0.05mg/mL.[QAPHELA-Esi sisombululo sinogxininiso olulingana ne-0.5% yaleyo yeSampuli yesisombululo.]
Isisombululo sesampuli: Dlulisa i-0.1 g yeTyrosine kwi-flask ye-volumetric ye-10-mL, yongeza isisombululo esaneleyo se-Diluted ammonia ukuze uchithe, kwaye uhlambulule ngamanzi ukuya kwivolumu.
Inkqubo yeChromatographic (Jonga iChromatography <621>, iChromatography eThin-Layer.)
Indlela: TLC
I-Adsorbent: 0.25-mm umaleko we-chromatographic umxube we-silica gel
Umthamo wesicelo: 5μL
Ukuphuhlisa inkqubo yokunyibilikisa: Isopropyl alcohol kunye neammonium hydroxide (7:3)
I-reagent yokutshiza: 2 mg/mL ye-ninhydrin kumxube we-butyl alcohol kunye ne-2N acetic acid (95:5)
Ukufaneleka kwenkqubo
Iimfuno zokufaneleka: Ichromatogram yesisombululo sokufaneleka kweSistim ibonisa amabala amabini ahluke ngokucacileyo.
Uhlalutyo
Iisampulu: isisombululo sokufaneleka kweNkqubo, isisombululo esiPhakathi, kunye nesisombululo seSampuli.
Yomisa ipleyiti phakathi kwe-100 ° kunye ne-105 ° de i-ammonia iphele ngokupheleleyo.Tfutha nge-Reagent yokutshiza, kunye nobushushu phakathi kwe-100 ° kunye ne-105 ° i-15 min.Hlola ipleyiti phantsi kokukhanya okumhlophe.
Iikhrayitheriya zokumkela: Nayiphi na indawo yesibini yesisombululo seSampuli ayinkulu okanye inamandla ngaphezu kwendawo eyintloko yesisombululo soMgangatho.
Nakuphi na ukungcola komntu: NMT 0.5%
Ubumdaka bubonke: NMT 2.0%
IIMVAVANYO EZITHILE
UKUJONGANA KOMBONISO, Ukujikeleza okuthe ngqo <781S>
Isisombululo sesampuli: 50 mg / mL kwi-1 N hydrochloric acid
Indlela yokwamkela: -9.8 ° ukuya -11.2 °
I-LASSON EKOMISI <731>: Yomisa isampuli kwi-105℃ kangange-3 h: ilahlekelwa yi-NMT nge-0.3% yobunzima bayo.
IIMFUNO EZONGEZELELWEYO
UKUPAKISHWA NOKUGCINWA: Gcina kwizikhongozeli ezivalwe kakuhle.
IMIGANGATHO YE-USP REFERENCE <11>
USP L-Phenylalanine RS
USP L-Tyrosine RS