Chenodeoxycholic Acid (CDCA) CAS 474-25-9 Ayẹwo ≥98% (Ipilẹ Gbẹ)

Chenodeoxycholic Acid
C24H40O4: 392.57
3a,7a-Dihydroxy-5b-cholan-24-oic acid [474-25-9]
Chenodeoxycholic Acid, nigbati o ba gbẹ, ko kere ju 98.0z ko si ju 101.0% ti C24H40O4 lọ.
Apejuwe Chenodeoxycholic Acid waye bi funfun, awọn kirisita, lulú okuta tabi lulú.
O jẹ tiotuka larọwọto ninu kẹmika ati ni ethanol (99.5), tiotuka ninu acetone, ati pe ko ṣee ṣe ninu omi.
Idanimọ Ṣe ipinnu iwoye gbigba infurarẹẹdi ti Chenodeoxycholic Acid, ti o gbẹ tẹlẹ, bi a ti ṣe itọsọna ni ọna disiki bromide potasiomu labẹ Infurarẹẹdi Spectrophotometry <2.25>, ki o si ṣe afiwe spekitiriumu pẹlu Spectrum Reference: mejeeji spectra ṣe afihan awọn inkikan gbigba ti o jọra ni awọn nọmba igbi kanna.
Yiyi opitika <2.49> [a] 20D: +11.0 °~ +13.0° (lẹhin gbigbe, 0.4 g, ethanol (99.5), 20 milimita, 100 mm).
Yiyọ ojuami <2.60> 164 ~ 169 ℃
Purity (1) Chloride <1.03> - Tu 0.36 g ti Chenodeoxycholic Acid ni 30 milimita kẹmika ti kẹmika, fi 10 milimita dilute nitric acid ati omi lati ṣe 50 milimita, ki o si ṣe idanwo pẹlu ojutu yii.Ṣetan ojutu iṣakoso bi atẹle: Si 1.0 milimita ti 0.01 mol / L hydrochloric acid VS fi 30 milimita ti methanol, 10 mL ti dilute nitric acid ati omi lati ṣe 50 milimita (kii ṣe ju 0.1%).
(2) Awọn irin eru <1.07>-Tẹsiwaju pẹlu 1.0 g ti Chenodeoxycholic Acid ni ibamu si Ọna 4, ati ṣe idanwo naa.Mura ojutu iṣakoso pẹlu 2.0 milimita ti Solusan Lead Standard (kii ṣe ju 20 ppm).
(3) Barium-Si 2.0 g ti Chenodeoxycholic Acid fi 100 milimita omi kun, ati sise fun awọn iṣẹju 2.Si ojutu yii ṣafikun 2 milimita ti hydrochloric acid, sise fun iṣẹju 2, ṣe àlẹmọ lẹhin itutu agbaiye, ki o wẹ àlẹmọ pẹlu omi titi yoo fi gba 100 milimita ti filtrate.Si 10 milimita ti filtrate ṣafikun 1 milimita ti sulfuric acid dilute: ko si turbidity ti han.
(4) Awọn nkan ti o jọmọ-tu 0.20 g ti Chenodeoxycholic Acid ni adalu acetone ati omi (9: 1) lati ṣe deede 10 milimita, ati lo ojutu yii bi ojutu ayẹwo.Lọtọ, tu 10 miligiramu ti lithocholic acid fun chromatography tinrin-Layer ninu adalu acetone ati omi (9: 1) lati ṣe deede 10 milimita.Pipet 2 milimita ti ojutu yii, ṣafikun adalu acetone ati omi (9: 1) lati ṣe deede 100 milimita, ati lo ojutu yii bi ojutu boṣewa (1).Lọtọ, tu 10 miligiramu ti ursodeoxycholic acid ninu adalu acetone ati omi (9: 1) lati ṣe deede 100 milimita, ati lo ojutu yii bi ojutu boṣewa (2).Lọtọ, tu 10 miligiramu ti cholic acid fun chromatography tinrin-Layer ninu adalu acetone ati omi (9: 1) lati ṣe deede 100 milimita, ki o lo ojutu yii bi ojutu boṣewa (3).Pipet 1 milimita ti ojutu ayẹwo, ki o ṣafikun adalu acetone ati omi (9: 1) lati ṣe deede 20 milimita.Pipet 0.5 milimita, 1 milimita, 2 milimita, 3 milimita ati 5 milimita ti ojutu yii, ṣafikun adalu acetone ati omi (1) si ọkọọkan wọn lati ṣe deede 50 milimita, ati ṣe apẹrẹ awọn ojutu wọnyi bi ojutu boṣewa A, ojutu boṣewa B, ojutu boṣewa C, ojutu boṣewa D ati ojutu boṣewa E, lẹsẹsẹ.Ṣe idanwo naa pẹlu awọn solusan wọnyi bi a ti ṣe itọsọna labẹ Chromatography tin-Layer<2.03>.Aami 5 milimita kọọkan ti ojutu ayẹwo, awọn ojutu boṣewa (1), (2), (3) ati awọn solusan boṣewa A, B, C, D ati E lori awo ti gel silica fun chromatography tinrin-Layer.Dagbasoke awo pẹlu adalu 4-methyl-2-pentanone, toluene ati formic acid (16: 6: 1) si ijinna ti o to 15 cm, gbẹ awo naa, ki o si gbẹ siwaju sii ni 1209C fun ọgbọn išẹju 30.Lẹsẹkẹsẹ, fun sokiri boṣeyẹ ojutu ti phosphomolybdic acid n-hydrate ni ethanol (95) (1 ni 5) lori awo, ati ooru ni 120 ℃ fun awọn iṣẹju 2 si 3: aaye ti o baamu aaye pẹlu ojutu boṣewa (1) ko lagbara ju aaye ti o ni ojutu boṣewa (1), aaye ti o baamu si aaye pẹlu ojutu boṣewa (2) ko ni iwọn diẹ sii ju aaye ti o ni ojutu boṣewa (2), ati aaye ti o baamu si aaye naa. pẹlu boṣewa ojutu (3) ni ko siwaju sii intense ju awọn iranran pẹlu bošewa ojutu
(3).Bi akawe si awọn aaye pẹlu awọn ojutu boṣewa A, B, C, D ati E, awọn aaye miiran yatọ si aaye akọkọ ati awọn aaye miiran ju awọn aaye ti a mẹnuba loke ko lagbara ju aaye pẹlu ojutu boṣewa E, ati iye lapapọ. ninu wọn ko ju 1.5%.
Pipadanu lori gbigbe <2.41> Ko siwaju sii ju 1.5% (1 g, 105 ℃, wakati 3).
Aloku lori ina <2.44> Ko ju 0.1% (1 g).
Assay Ṣe iwọn deede nipa 0.5 g ti Chenodeoxycholic Acid, ti o gbẹ tẹlẹ, tu ni 40 milimita ethanol (95) ati 20 milimita omi, ati titrate <2.50> pẹlu 0.1 mol/L sodium hydroxide VS (titration potentiometric).Ṣe ipinnu òfo ni ọna kanna, ati ṣe atunṣe to ṣe pataki.
milimita kọọkan ti 0.1 mol/L sodium hydroxide VS ＝ 39.26 mg ti C24H40O4
Awọn apoti ati awọn apoti ipamọ-Awọn apoti ti o nipọn.