Phthalimide Potassium Iyọ CAS 1074-82-4 Mimọ>99.0% (HPLC)

3. Ọna idanwo
3.1 Pipadanu lori Gbigbe (W/W)
3.1.1 Awọn ẹrọ ati awọn ohun elo
Ọkan ju ẹgbẹrun mẹwa ti iwọntunwọnsi itanna, adiro gbigbẹ thermostatic ina, igo wiwọn 40*25, ẹrọ gbigbẹ
Reagent: jeli siliki
3.1.2 Awọn ọna
1. Mọ 3 40 * 25 igo iwọn.
2, fi sinu adiro ni 105 ℃ (± 2 ℃) gbẹ fun wakati kan.
3. Mu igo wiwọn 40 * 25 jade ki o si fi sinu ẹrọ gbigbẹ lati tutu fun 30min.
4, Iwọn wiwọn deede 40 * 25 igo wiwọn iwuwo ṣofo W0.
5. Lo igo iwuwo 40 * 25 lati ṣe iwọn ayẹwo ti o dapọ deede ti 3.0000g (W1), lapapọ 3.
6, Fi sinu adiro, yọ ideri igo naa kuro, fi sii lẹgbẹẹ igo wiwọn, tabi idaji ṣii igo igo fun gbigbẹ, mu jade, gbọdọ jẹ iwọn igo ti o dara, gbẹ ni 105 ℃ (± 2℃) fun wakati 3.
7. Mu igo ti o ni iwọn 40 * 25 jade ki o si fi sinu ẹrọ gbigbẹ lati dara fun 30min.
8, Iwọn deede lẹhin gbigbe 40 * 25 iwuwo ti igo W2.
Ilana Iṣiro: Pipadanu lori Gbigbe (%) = (W1-W2)/(W1-W0)×100%
3. 4 Ipinnu akoonu
3.4.1 Irinse ati ẹrọ itanna
LC-UV100 Liquid Chromatograph (Shanghai Wufeng Scientific Instrument Co., LTD.)
10ml volumetric igo, irin alagbara, irin igun sibi ati awọn miiran jẹmọ awọn ohun elo
3.3.2 Awọn ọna
Ọna fun itupalẹ Phthalimide Potassium Iyọ
3.3.3 Liquid kiromatogirafi
Chromatographic majemu
Iwe Chromatographic: HypersilC6H5 (C8 tun wa) 4.6*250mm 5um
Wifulfulenti: 210nm
Iwọn otutu: 30 ℃
Oṣuwọn sisan: 1.0ml/min
Idojukọ ayẹwo: 0.05mg/ml (diluent jẹ alakoso alagbeka)
Reagents
Potasiomu Phosphate Dibasic(itupalẹ mimọ)
Phthalimide Potassium iyọ (apẹẹrẹ boṣewa)
Acetonitrile (itupalẹ mimọ)
Omi funfun
Mobile alakoso: acetonitrile: fosifeti saarin (PH: 3.5) = 40:60
Ṣe iwọn 1.63g Potassium Phosphate Dibasic ki o tu ni 600ml omi mimọ, ṣatunṣe iye PH si 3.5 pẹlu phosphoric acid, tú sinu beaker 1000ml ti o ni 400ml acetonitrile, dapọ daradara, àlẹmọ pẹlu 0.45um adalu okun àlẹmọ okun, tu ati imurasilẹ pẹlu gbigbọn ultrasonic. ohun elo.
3.3.4 Ilana
1) Ojutu idanwo atunto (0.05mg/ml)
Ayẹwo 2.5mg naa ni iwọn deede ati fi sinu igo iwọn didun 50ml kan.A ṣe afikun alakoso alagbeka ati ti fomi si iwọn nipasẹ itujade ultrasonic.
2) Ojutu boṣewa iṣeto ni (1.0mg/ml)
Ayẹwo boṣewa 2.5mg ti ni iwọn deede ati fi sinu igo iwọn didun 50ml kan.A ṣe afikun alakoso alagbeka ati ti fomi si iwọn nipasẹ itujade ultrasonic.
3) Lilo eto:
(1) Fi ori àlẹmọ sinu 80% methanol ni ibẹrẹ, ṣii àtọwọdá eefi ati sisan fun 3-5min, ki o si pa àtọwọdá eefin naa ki o fi omi ṣan fun 10-15min.Lẹhinna fi ori àlẹmọ sinu 90% omi mimọ, ṣii àtọwọdá sisan ati ṣiṣan fun 3-5min, pa àtọwọdá sisan naa ki o fi omi ṣan fun 10min.
(2) Lẹhinna yi ipele alagbeka ti a pese silẹ, iwọn sisan jẹ 1.0ml / min, titẹ iwaju iwe ko kere ju 1000Psi, ṣii oluwari, duro titi titẹ iwaju iwaju iwe jẹ iduroṣinṣin, ṣii iṣẹ kọnputa, ati tẹ ori ayelujara
(3) Lẹhin titẹ iwaju ọwọn jẹ iduroṣinṣin, ṣii oluwari naa.Ni akọkọ abẹrẹ 1 ti alakoso alagbeka, lẹhinna fun abẹrẹ 1 ti apẹẹrẹ boṣewa, ati nikẹhin itasi ayẹwo.
(4) Awọn abajade iṣiro: ọna deede agbegbe
(5) Lẹhin ti pari iṣẹ-ṣiṣe onínọmbà, pa aṣawari akọkọ, lẹhinna fi omi ṣan ori àlẹmọ ni omi mimọ fun 10-15min;Ni akoko kanna, abẹrẹ abẹrẹ ti a fi omi ṣan pẹlu omi ati kẹmika.
(6) Níkẹyìn, fi methanol 80% wọ, fi omi ṣan fun 30min, ki o si pa.
4. Lẹhin ipari ti ayewo, fọwọsi ni ayewo ati iwe idanwo yàrá.