I-5-Fluorocytosine (5-FC) CAS 2022-85-7 Purity ≥99.5% (HPLC) I-Capecitabine Emtricitabine Intermediate Factory

I-5-Fluorocytosine (5-FC) CAS: 2022-85-7 Indlela Yokuhlaziya
Ukubukeka-Okumhlophe noma Okucishe Kube I-Crystalline Powder Emhlophe
Ukulahlekelwa Ekusuleni-Yomise ku-105 amahora angu-4: ayilahlekelwa ngaphezu kuka-1.50% wesisindo sayo.
Insalela Ekushiseni-hhayi ngaphezu kuka-0.1%.
I-Heavy Metals - hhayi ngaphezu kuka-0.002%.
I-Fluoride-Hlanganisa u-70 mL wesixazululo esisanda kulungiswa se-potassium chloride esigcwele esino-30 ml we-isopropyl alcohol, gcwalisa i-electrode nge-supernatant ecacile, bese uvumela i-electrode ukuthi ihlale kule ngxube amahora angekho ngaphansi kwama-2 ngaphambi kokusetshenziswa, noma okungcono kakhulu ebusuku.
Lapho uthatha izilinganiso, dlulisela isixazululo kubhekhakhi engu-150-mL, bese ucwilisa ama-electrode.Faka ibha eshukumisayo enama-polytef kubhekha, beka ibhekha kusivusi kazibuthe esinendawo engaphezulu evalekile, bese uvumela ukunyakazisa kuze kufinyelelwe ukulingana (cishe imizuzu engu-1 ukuya kwemi-2).Hlanza futhi womise ama-electrode phakathi kwezilinganiso, uqaphele ukuthi ungaklwebheli ikristalu ku-electrode ye-ion ethile.
Linganisa amandla okulungiselelwa Okujwayelekile ngakunye, bese uhlela ukugxila kwe-fluoride, ngo-mg nge-100 mL ngayinye, ngokuqhathanisa namandla, ku-mV, ephepheni le-semilogarithmic.Linganisa amandla okulungiselela Ukuhlolwa, bese unquma ejikeni elijwayelekile ukugcwala kwe-fluoride, ngo-mg nge-100 ml ngayinye.Bala iphesenti le-fluoride engxenyeni ye-Flucytosine ethathwe ifomula:
C / 10
lapho u-C ewukugxiliswa kwe-fluoride, ku-mg ku-100 mL, kusukela kwijika elijwayelekile: akutholakali ngaphezu kuka-0.05% we-fluoride.
I-Fluorouracil-Dissolve 250 mg ku-10 mL wengxube ye-glacial acetic acid namanzi (4:1).Faka u-20 µL walesi sixazululo epuletini le-chromatographic lisendlalelo oluncane olunamekwe ngesendlalelo esingu-0.5-mm sengxube yejeli ye-chromatographic.Epuletini elifanayo sebenzisa u-20 µL, ekunyusweni okungu-10-µL, ka-0.025 mg ngesixazululo se-mL se-USP Fluorouracil RS kungxube ye-glacial acetic acid namanzi (4:1).Yakha i-chromatogram ngengxube ye-chloroform ne-glacial acetic acid (13:7) kuze kube yilapho i-solvent yangaphambili isuka endaweni engekho ngaphansi kuka-14 cm ukusuka lapho ivela khona.Susa ipuleti ekamelweni elikhulayo, futhi uvumele i-solvent ihwamuke.Thola amachashaza epuletini ngokubheka ngaphansi kwemisebe ye-UV yamaza amafushane: noma iyiphi indawo evela esixazululweni esivivinywayo ayinkulu ngosayizi nokuqina kunendawo eku-RF efanelekile ekhiqizwe isixazululo Esijwayelekile, esihambisana nokungadluli ku-0.1% we i-fluorouracil.
I-Assay-Place cishe u-400 mg we-Flucytosine, ukalwe ngokunembile, ebhekhani elingu-250-mL, engeza u-150 mL wengxube yevolumu engu-2 ye-glacial acetic acid kanye nevolumu engu-1 ye-acetic anhydride, bese uncibilikisa, ufudumale kancane uma kunesidingo.Titrate potentiometrically nge-0.1 N perchloric acid VS, usebenzisa isistimu ye-electrode yengilazi ye-calomel.Yenza isinqumo esingenalutho, futhi wenze noma yikuphi ukulungisa okudingekayo.I-mL ngayinye ka-0.1 N perchloric acid ilingana no-12.91 mg we-C4H4FN3O.I-Flucytosine iqukethe amaphesenti angekho ngaphansi kwamaphesenti angu-98.5 futhi angabi ngaphezu kwamaphesenti angu-101.0 we-C4H4FN3O, abalwe ngokomisiwe.