I-Acesulfame K CAS 55589-62-3 Acesulfame Potassium Purity >99.0% (HPLC)

INCAZELO
I-Acesulfame Potassium iqukethe i-NLT 99.0% kanye ne-NMT 101.0% ye-C4H4NO4SK, ibalwe ngokomisiwe.
UKUKHOMBA
• A. UKUMUMVA OKUNOMTHWALO <197K>
• B. IZIVIVINYO ZOKUKHOMBA JIKELELE, I-Potassium <191>
Isixazululo sesampula: 100 mg/mL
Indlela yokwamukela: Ihlangabezana nezimfuneko
ISIVIVINYO
• INQUBO
Isampula: 150 mg
Isistimu ye-Titrimetric (Bona i-Titrimetry <541>)
Imodi: I-titration eqondile
I-Titrant: 0.1 N i-perchloric acid VS
Okungenalutho: 50 mL we-glacial acetic acid
Ukutholwa kwendawo yokugcina: I-Potentiometric
Ukuhlaziya: Chaza Isampula ku-50 mL we-glacial acetic acid.
Titrate ngo-0.1 N perchloric acid VS.Yenza isinqumo esingenalutho.
Bala iphesenti le-acesulfame potassium (C4H4NO4SK) Kusampula:
Umphumela = [(V − B) × N × F × 100]/W
V = ivolumu ye-titrant esetshenziswe Isampula (mL)
B = ivolumu ye-titrant edliwe yi-Blank (mL)
N = ukujwayela kwangempela kwe-titrant (mEq/mL)
F = i-equivalency factor, 201.2 mg/mEq
W = isisindo sesampula (mg)
Indlela yokwamukela: 99.0%–101.0% ngokomisiwe
UKUNGCOLA
• UMKHAWULO WE-FLUORIDE
[QAPHELA-Sebenzisa izinto zepulasitiki kukho konke lokhu kuhlolwa.]
Isixazululo A: Chaza u-210 g we-citric acid monohydrate ku-400 mL wamanzi.Lungisa nge-ammonia egxilile ibe yi-pH engu-7.0, bese uhlanza ngamanzi abe ngu-1000 mL.
Isixazululo B: 132 mg/mL ye-dibasic ammonium phosphate
Isixazululo C: Ekumisweni okungu-292 g we-edetic acid ku-500 mL wamanzi, engeza u-200 mL we-ammonium hydroxide, ulungise ne-ammonium hydroxide ku-pH ephakathi kuka-6 no-7, bese uhlanza ngamanzi ukuze wenze u-1000 mL.
Isixazululo se-buffer: Hlanganisa amavolumu alinganayo e-Solution A, Isixazululo B ne-Solution C, bese ulungisa nge-ammonium hydroxide ku-pH engu-7.5.
Isixazululo esijwayelekile sesitoko: Kala u-0.442 g we-sodium fluoride, omiswe ngaphambilini ku-300° amahora angu-12, ube yi-1-L ye-volumetric flask, bese uhlanjululwa ngamanzi ngevolumu.Gcina isixazululo esitsheni sepulasitiki esivaliwe.Ngokushesha ngaphambi kokusetshenziswa, i-pipet 5 mL yalesi sixazululo ku-100-mL ye-volumetric flask, bese uhlanza ngamanzi ngevolumu.I-mL ngayinye yalesi sixazululo iqukethe u-10µg we-ion fluoride.
Isixazululo esijwayelekile A: Xuba u-0.5 mL wesixazululo sesitoko Esijwayelekile kanye no-15.0 mL wekhambi le-Buffer, bese uxuba ngamanzi ku-50mL.
Isixazululo esijwayelekile B: Xuba u-1.0 mL wesisombululo sesitoko Esijwayelekile kanye no-15.0 mL wekhambi le-Buffer, bese uxuba ngamanzi ku-50mL.
Isixazululo esijwayelekile C: Xuba u-1.5 mL wesisombululo sesitoko Esijwayelekile kanye no-15.0 mL wekhambi le-Buffer, bese uxuba ngamanzi ku-50mL.
Isixazululo esijwayelekile D: Xuba u-3.0 mL wesisombululo sesitoko Esijwayelekile kanye no-15.0 mL wekhambi le-Buffer, bese uxuba ngamanzi ku-50mL.
Isixazululo esiyisampula: Ku-50-mL ye-volumetric flask engeza u-3 g we-Acesulfame Potassium.Chaza emanzini, engeza u-15.0 mL wekhambi le-Buffer, bese uhlanza ngamanzi ngevolumu.
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile A, Isixazululo esijwayelekile B, isixazululo esijwayelekile C, isixazululo esijwayelekile D, kanye nesixazululo esiyisampula
Kala kanyekanye amandla (bona i-Titrimetry <541>) ku-mV, yezixazululo Ezijwayelekile kanye nesisombululo Sesampuli, imitha ye-pH efanelekile efakwe i-electrode eqondene ne-fluoride kanye ne-silver-silver chloride reference electrode.Lapho uthatha izilinganiso, dlulisela isixazululo kubhekha we-25-mL, bese ucwilisa ama-electrode.Faka ibha eshukumisayo enamekwe nge-polytef kubhekha, beka ibhekha kusishukumisi kazibuthe esinendawo engaphezulu evalekile bese uvumela ukunyakazisa kuze kube yilapho kufinyelelwa ukulingana (1-2 min).Hlanza, futhi womise ama-electrode phakathi kwezilinganiso, uqaphele ukuthi ungaklwebheli ikristalu ku-electrode ye-ion eqondene ne-fluoride.Linganisa amandla esixazululo ngasinye Esijwayelekile, bese uhlela ukugxila kwe-fluoride, ku-µg/mL, ngokuqhathanisa namandla, nge-mV, ephepheni le-semilogarithmic.Linganisa amandla esixazululo Sesampuli, bese unquma ukugxiliswa kwe-fluoride ukusuka kwijika elijwayelekile, ku-µg/mL.
Bala okuqukethwe, ngo-ppm, kwe-fluoride engxenyeni ye-Acesulfame Potassium ethathiwe:
Umphumela = (V × C/W)
V = umthamo wesixazululo sesampula (mL)
C = ukugcwala kwe-fluoride kusixazululo Sesampula, kusukela kujika elijwayelekile (mg/mL)
W = isisindo se-Acesulfame Potassium esithathwe ukulungisa Isampula yesixazululo (g)
Imibandela yokwamukela: NMT 3 ppm
• IZIMTHETHO EZISINDAYO, Indlela I <231>: NMT 10 ppm
• UKWETHEMBEKA KWE-CHROMATOGRAPHIC
Isixazululo A: 3.3 mg/mL ye-tetrabutylammonium hydrogen sulfate
Isigaba seselula: I-Acetonitrile ne-Solution A (2:3)
Isixazululo sokufaneleka kwesistimu: 2 µg/mL ngayinye ye-USP Acesulfame Potassium RS ne-ethylparaben
Isixazululo esijwayelekile: 0.2 µg/mL ye-USP Acesulfame Potassium RS
Isixazululo sesampula: 10 mg/mL
Isistimu yeChromatographic
(Bona i-Chromatography <621>, Ukufaneleka Kwesistimu.)
Imodi: LC
Umtshina: UV 227 nm
Ikholomu: 4.6-mm × 25-cm;5-µm ukupakisha i-L1
Izinga lokugeleza: 1 mL/min
Usayizi womjovo: 20 µL
Ukufaneleka kwesistimu
Isampula: Isixazululo sokufaneleka kwesistimu
Izidingo zokufaneleka
Isixazululo: I-NLT 2 phakathi kwe-acesulfame potassium ne-ethylparaben
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile kanye nesixazululo esiyisampula
Rekhoda ama-chromatogram ngesikhathi sokugijima i-NLT izikhathi ezi-3 zokugcinwa kwe-acesulfame potassium peak, futhi ulinganise izimpendulo zendawo zeziqongo.
Indlela yokwamukela: Ukusabela kwanoma yikuphi ukuphakama ngesikhathi sokugcinwa ngaphandle kwalokho kwe-acesulfame potassium evela kusixazululo Sesampuli ayeqi impendulo ye-acesulfame potassium peak kusukela kusixazululo Esijwayelekile (0.002%).
IZIVIVINYO EZITHILE
• UBUSIDI NOMA UBUNGOTI
Isixazululo sesampula: 4.0 g ku-20 mL wamanzi angenayo i-carbon dioxide
Ukuhlaziywa: Engeza u-0.1 mL we-bromothymol blue TS.Uma isixazululo siphuzi, titrate nge-0.01 N sodium hydroxide ukukhiqiza umbala oluhlaza okwesibhakabhaka.Uma isixazululo siluhlaza okwesibhakabhaka, titrate nge-0.01 N hydrochloride acid ukukhiqiza umbala ophuzi.
Imibandela yokwamukela: I-NMT 0.2 mL ka-0.01 N sodium hydroxide noma i-NMT 0.2 mL ka-0.01 N hydrochloric acid iyadingeka.
• UKULAHLEKELWA EKOMISENI <731>: Yomisa isampula ku-105° amahora angu-3: ilahlekelwa yi-NMT 1.0% yesisindo sayo.
IZIDINGO EZENGEZIWE
• UKUPAKASHA KANYE NESIGCINI: Londoloza esitsheni esivaleke kahle, futhi uvikele ekukhanyeni.Gcina ekamelweni lokushisa.
I-USP REFERENCE AMAZINGA <11>
USP Acesulfame Potassium RS