I-Adenine CAS 73-24-5 Assay 98.0%~102.0% (Titration) Imboni Yokuhlanzeka Okuphezulu

I-Adenine iqukethe i-NLT 98.0% kanye ne-NMT 102.0% ye-C 5H5N5, ibalwe ngokomisiwe.
UKUKHOMBA
A. UKUMUMVA OKUTHI INFRARED <197K>
I-ASSAY
INQUBO
Isampula: 200 mg we-Adenine
Akunalutho: 80 mL wengxube ye-100 ml ye-glacial acetic acid kanye nama-300 mL we-acetic anhydride
Titrimetric System
Imodi: I-titration eqondile
I-Titrant: 0.1 N nge-chloric acid ngayinye VS ifaneke kanjena: Dlulisa u-300 mg we-potassium biphthalate kubhekha elingu-150-mL futhi, ngokuhlanganisa, ncibilikisa ku-80 mL wengxube engu-100 mL ye-glacial acetic acid kanye no-300 mL we-acetic anhydride.Titrate ngesisombululo se-chloric acid ngasinye.U-20.42mg ngamunye we-potassium biphthalate ulingana no-1 mL ka-0.1 N perchloric acid.
Ukutholwa kwendawo yokugcina: I-Potentiometric
Ukuhlaziya: Chaza Isampula ku-80 mL wengxube engu-100 mL ye-glacial acetic acid kanye nama-300 mL we-acetic anhydride ngokugoqoza, kanye ne-titrate ne-Titrant.Bala iphesenti le-Adenine (C5H5N5) engxenyeni ethathiwe:
Umphumela = [(V − B) × N × F × 100]/W
V = Isampula yevolumu ye-titrant (mL)
B = Ivolumu ye-titrant engenalutho (mL)
N = ukujwayelekile kwe-titrant (mEq/mL)
F = i-equivalency factor, 135.13 mg/mEq
W = isisindo sesampula (mg)
Indlela yokwamukela: 98.0%–102.0% ngokomisiwe
UKUNGCOLA
Ukungcola Okungaphili
UKUSHISA OKUSELELE <281>: NMT 0.1%
IZINSIMBI EZISINDAYO, Indlela II <231>: NMT 10ppm
Ukungcola Okuphilayo
INQUBO
i-pH 7.0 i-phosphate buffer: Chaza u-4.54 g we-monobasic potassium phosphate emanzini ukwenza u-500 mL wesisombululo.Chaza u-4.73 g we-anhydrous dibasic sodium phosphate emanzini ukwenza u-500 mL wesixazululo.Hlanganisa u-38.9 mL wesisombululo se-monobasic potassium phosphate no-61.1 mL wesisombululo se-dibasic sodium phosphate.Lungisa, uma kunesidingo, ngokufaka i-dropwise yesisombululo se-dibasic sodium phosphate sibe yi-pH engu-7.0.
Isixazululo sesitoko esijwayelekile: Chaza inani elifanelekile le-USP Adenine RS emanzini ashisayo, apholile, futhi uhlambulule ngobuningi ngamanzi ukuze uthole isixazululo esinokugxila okwaziwayo okungu-0.19 mg/mL.
Izisombululo ezijwayelekile: Ipayipi elingu-5-mL izingxenye zesixazululo sesitoko Esijwayelekile kumaflask amathathu evolumu angu-100-mL, futhi axutshwe ne-0.10 N hydrochloric acid, 0.010 N sodium hydroxide, kanye ne-pH 7.0 phosphate buffer, ngokulandelana, kuvolumu.
Isixazululo sesitoko esiyisampula: Chaza inani elifanelekile le-Adenine emanzini ashisayo, epholile, futhi uhlambulule ngobuningi ngamanzi ukuze uthole isixazululo esinokugxila okwaziwayo okungu-0.19 mg/mL.
Isixazululo sesitoko esiyisampula: Chaza inani elifanelekile le-Adenine emanzini ashisayo, epholile, futhi uhlambulule ngobuningi ngamanzi ukuze uthole isixazululo esinokugxila okwaziwayo okungu-0.19 mg/mL.
Izisombululo zesampula: Ipayipi elingu-5-mL izingxenye zesixazululo sesitoko sesampula kumaflask amathathu evolumu angu-100-mL, futhi axutshwe ngo-0.10 N hydrochloric acid, 0.010 N sodium hydroxide, kanye ne-pH 7.0 phosphate buffer, ngokulandelana, kuvolumu.
Izimo ze-Spectrometric
(Bheka i-Spectrophotometry ne-Light-scattering <851>.)
Imodi: UV-Vis
Ibanga le-wavelegth: 220–320 nm
Iseli: 1 cm
Okungenalutho: Amanzi
Ukuhlaziya
Amasampula: Izixazululo ezijwayelekile kanye nezixazululo zesampula
Imibandela yokwamukela: Ukumuncwa okulandelanayo, okubalwe ngokomisiwe, kumaza wamaza okumunca kakhulu, kupheya ngalinye lezixazululo ezihambisanayo awehluki ngaphezu kuka-2.0%.
IZIVIVINYO EZITHILE
I-LASSON DRYING <731>: Yomisa isampula ku-110 ° amahora angu-4: ilahlekelwa i-NMT 1.0% yesisindo sayo.
OKUQUKETHWE I-NITROGEN, Indlela II 〈461〉: 50.2%–53.4%, ibalwa ngesisekelo esomisiwe
IZIDINGO EZENGEZIWE
UKUPAKASHA KANYE NESIGCINI: Londoloza ezitsheni ezivalwe kahle.
I-USP REFERENCE AMAZINGA <11>
USP Adenine RS