I-Adenosine CAS 58-61-7 Assay 99.0% -101.0% USP Standard Factory High Purity

INCAZELO
I-Adenosine iqukethe i-NLT 99.0% kanye ne-NMT 101.0% ye-C10H13N5O4, ibalwe ngokomisiwe.
UKUKHOMBA
• A. UKUMUMVA OKUNOMTHETHO <197K>: NMT 0.1%
I-ASSAY
• I-ADENOSINE
Isampula: 200 mg we-Adenosine omiswe ngaphambilini ku-105° amahora angu-2
Isistimu ye-Titrimetric
(Bheka i-Titrimetry <541>)
Imodi: I-titration eqondile
I-Titrant: 0.1 N nge-chloric acid VS
Ukutholwa kwendawo yokugcina: I-Potentiometric
Ukuhlaziywa: Chaza ku-50 mL we-glacial acetic acid kanye ne-titrate ngo-0.1 N nge-chloric acid ngayinye VS.Bala iphesenti le-Adenosine (C10H13N5O4) engxenyeni ethathiwe:
Umphumela = [(V − B) × N × F × 100]/W
V = Isampula yevolumu ye-titrant (mL)
B = Ivolumu ye-titrant engenalutho (mL)
N= okujwayelekile kwe-titrant (mEq/mL)
F= i-equivalency factor: 267.25 mg/mEq
W= isisindo Sesampula (mg)
Indlela yokwamukela: 99.0% -101.0% ngokomisiwe
• Ukungcola
• UKUSHISA KWESALELA <281>: NMT 0.1%
• IZIMTHETHO EZISINDAYO, Indlela II <231>: NMT 10 ppm
• UMKHAWULO WE-AMMONIA
Isixazululo sesampula: Misa u-0.5 g ku-10 mL wamanzi.Govuza imizuzwana engu-30, bese udlula ngesihlungi esimahhadla.Nciphisa i-filtrate ngamanzi ku-15 mL, bese usebenzisa i-filtrate.
Isixazululo esijwayelekile: 0.4 µg/mL ye-ammonium chloride emanzini
Ukuhlaziya: Kusixazululo Sesampula kanye nekhambi Elijwayelekile engeza u-0.3 mL we-alkaline mer curic–potassium iodide TS, vala amashubhu okuhlola, bese uvumela ukuthi ime imizuzu emi-5.
Imibandela yokwamukela: Isixazululo Sesampula asibonisi umbala ophuzi oqine kakhulu kunalowo Wesixazululo Esijwayelekile (NMT 4 ppm of ammonia).
• UMKHAWULO WE-CHLORIDE
Isixazululo sesampula: Misa u-0.2 g ku-10 mL wamanzi.Govuza imizuzwana engu-30, udlule kusihlungi esimahhadla, bese usebenzisa i-filtrate.
Isixazululo esijwayelekile: 2.3 µg/mL ye-sodium chloride emanzini
Ukuhlaziya: Kusixazululo Sesampula kanye no-10 mL wesixazululo Esijwayelekile engeza u-1 mL we-nitric acid no-1 mL we-nitrate yesiliva engu-TS, bese uhlanza isisombululo ngasinye ngamanzi sibe ngu-40 mL.Vumela izixazululo zime imizuzu engu-5, zivikelekile ekukhanyeni.
Imibandela yokwamukela: Uma ibhekwa kungemuva elimnyama, isixazululo Sesampuli asibi kakhulu kunesixazululo Esijwayelekile (NMT 0.007% chloride).
• UMKHAWULO WE-SULFATE
Isixazululo sesampula: Misa u-0.75 g ku-15 mL wamanzi.Govuza imizuzwana engu-30, udlule kusihlungi esimahhadla, bese usebenzisa i-filtrate.
Isixazululo esijwayelekile: Engeza u-0.15 mL we-0.020 N sulfuric acid ku-15 mL wamanzi.
Ukuhlaziya: Kusixazululo Sesampula kanye nesixazululo Esijwayelekile engeza u-2 mL we-barium chloride TS no-1 mL we-3 N hydrochloric acid, hlambulula isixazululo ngasinye ngamanzi sibe ngu-30 mL, bese uxuba.Vumela izixazululo zime imizuzu emi-5.
Imibandela yokwamukela: Isixazululo Sesampula asinaso i-turbid kunesixazululo Esijwayelekile (NMT 0.02% sulfate).
• UKUNGCOLA KOKUPHILA
Isixazululo A: 6.8 g/L we-potassium hydrogen sulfate kanye no-3.4g/L we-tetrabutylammonium hydrogen sulfate emanzini.Lungisa nge-2 N potassium hydroxide uye ku-pH engu-6.5.
Isixazululo B: 0.1 g/L yesisombululo se-sodium azide
Isigaba seselula: Isixazululo A nesixazululo B (60:40)
Isixazululo sokufaneleka kwesistimu: 0.2 mg/mL ngayinye ye-Adenosine ne-inosine esigabeni Seselula
Isixazululo sesampula: 1.0 mg/mL ye-Adenosine esigabeni Seselula
Isistimu yeChromatographic
(Bona i-Chromatography <621>, Ukufaneleka Kwesistimu.)
Imodi: LC
Umtshina: UV 254 nm
Ikholomu: 4.6-mm × 25-cm;5-µm ukupakisha i-L1
Izinga lokugeleza: 1.5 mL/min
Usayizi womjovo: 20 µL
Ukufaneleka kwesistimu
Amasampula: Isixazululo sokufaneleka kwesistimu
Izidingo zokufaneleka
Ukulungiswa: I-NLT 9.0 phakathi kwe-adenosine ne-inosine
Isici somsila: NMT 2.5
Ukuchezuka okujwayelekile okuhlobene: NMT 2.0%
[QAPHELA-I-Chromatograph isixazululo Sesampula, futhi ulungise isikhathi sokusebenza sibe okungenani kabili isikhathi sokugcina senani eliphakeme kakhulu.]
Ukuhlaziya
Isampula: Isixazululo sesampula
Bala iphesenti lokungcola ngakunye engxenyeni ye-Adenosine ethathiwe:
Umphumela = (rU/rT) × 100
i-rU = impendulo ephakeme kakhulu yokungcola ngakunye okuvela kusixazululo Sesampula
rT = isamba sazo zonke izimpendulo zazo zonke iziqongo ezivela kusixazululo Sesampula
Imibandela yokwamukela
Ukungcola komuntu ngamunye: I-NMT 0.1% ngayinye ye-guanosine, inosine, ne-uridine, kanye ne-NMT 0.2% ye-adenine
Ukungcola okuphelele: NMT 0.5%
IZIVIVINYO EZITHILE
• UBUNINGI OKUNCIKAYO NOMA ITHEMPERESHA <741>: 233°-238°
• UKUZUNGUZA KWE-OPTICAL, Ukuzungezisa Okuqondile <781S>: -68° kuya -72°
Isixazululo sokuhlola: 20 mg/mL kusixazululo se-sodium hydroxide (1 ku-20), sinqunywa kusampula elomiswe ngaphambilini ku-105° amahora angu-2
I-acidity noma i-alkali: Misa u-1 g ku-20 mL wamanzi angenayo i-carbon dioxide.Govuza imizuzwana engu-30, bese udlula ngesihlungi esimahhadla.Ezingxenyeni ezimbili ze-10-mL ze-filtrate engeza u-0.1mL we-bromocresol purple TS.
Imibandela yokwamukela: I-NMT 0.3 mL ka-0.01 N sodium hydroxide iyadingeka ukukhiqiza umbala oluhlaza okwesibhakabhaka-violet engxenyeni eyodwa.I-NMT 0.1 mL ka-0.01 N hydrochloric acid iyadingeka ukukhiqiza umbala ophuzi kwenye ingxenye.
•I-LOSSON DRYING <731>: Yomisa isampula ku-105℃ amahora angu-2: ilahlekelwa yi-NMT ngo-0.5% wesisindo sayo.
IZIDINGO EZENGEZIWE
•UKUPAKASHWA KANYE NESIGCINI: Londoloza ezitsheni eziqinile, ezikwazi ukumelana nokukhanya, futhi ugcine endaweni elawulwayo yegumbi lokushisa.
• AMAZINGA EREferensi e-USP <11>
USP Adenosine RS