I-Citric Acid Anhydrous CAS 77-92-9 Assay 99.5~100.5%

I-Citric acid [77-92-9].
INCAZELO
I-Anhydrous Citric Acid iqukethe i-NLT 99.5% kanye ne-NMT 100.5% ye-C6H8O7, ebalwe ngesisekelo se-anhydrous.
UKUKHOMBA
• I-Infrared Absorption <197K>: Yomisa into ezohlolwa ku-105℃ amahora angu-2.
ISIVIVINYO
• Inqubo
Isampula: 0.550 g ye-Anhydrous Citric Acid;rekhoda isisindo ngokunembile.
Ukuhlaziya: Chaza Isampula ku-50 ml wamanzi.Engeza u-0.5 mL we-phenolphthalein TS.Titrate nge-1 N sodium hydroxide VS.I-mL ngayinye ye-1 N sodium hydroxide ilingana no-64.03 mg we-C6H8O7.
Indlela yokwamukela: 99.5% -100.5% ngesisekelo se-anhydrous
UKUNGCOLA
Ukungcola Okungaphili
• Insalela Ekuthutheni <281>: I-NMT 0.1%, inqunywa ku-1.0 g
• I-Heavy Metals <231>: NMT 10 ppm
• I-sulfate
Isixazululo esijwayelekile se-sulfate A: 1.81 mg/mL we-potassium sulfate ku-30% wotshwala.Ngokushesha ngaphambi kokusetshenziswa, dlulisela i-10.0 mL yalesi sixazululo ku-1000-mL ye-volumetric flask, hlambulula ngo-30% wotshwala kuvolumu, bese uxuba.Lesi sixazululo siqukethe u-10 µg/mL we-sulfate.
Isixazululo esijwayelekile se-sulfate B: 1.81 mg/mL ye-potassium sulfate emanzini.Ngokushesha ngaphambi kokusetshenziswa, dlulisela u-10.0 mL walesi sixazululo ku-1000-mL ye-volumetric flask, hlambulula ngamanzi ngevolumu, bese uxuba.Lesi sixazululo siqukethe u-10 µg/mL we-sulfate.
Isixazululo sesitoko esiyisampula: 66.7 mg/mL ye-citric acid
Isixazululo esiyisampula: Ku-4.5 mL we-Standard sulfate solution, engeza u-3 mL wengxube ye-barium chloride (1 ku-4), nyakazisa, bese uvumela ukuthi ime iminithi elingu-1.Ku-2.5 mL we-suspension ewumphumela, engeza u-15 mL weSample stock solution kanye no-0.5 mL we-5 N acetic acid, bese uxuba.
Isixazululo esijwayelekile: Lungiselela njengoba uqondiswe kwisixazululo Sesampula, ngaphandle kokusebenzisa u-15 mL we-Standard sulfate solution B esikhundleni sesixazululo sesitoko esiyisampula.
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile kanye nesixazululo esiyisampula
Imibandela yokwamukela: Noma yikuphi ukungcola okukhiqizwa kusixazululo Sesampuli ngemva kokuma kwamaminithi angu-5 akukukhulu kunalokho okukhiqizwa kusixazululo Esivamile (0.015%).
• Umkhawulo we-Aluminium (lapho ibhalwe ukuthi ihloselwe ukusetshenziswa ku-dialysis)
Isixazululo se-aluminium esijwayelekile: Ku-352 mg we-aluminium potassium sulfate ku-100-mL yevolumu ye-volumetric flask, engeza ama-mL ambalwa wamanzi, ujikeleze ukuze uhlakaze, engeza u-10 mL we-sulfuric acid ehlanjululwe, hlambulula ngamanzi ngevolumu, bese uxuba.Ngokushesha ngaphambi kokusetshenziswa, hlambulula u-1.0 mL walesi sixazululo ngamanzi abe ngu-100.0 mL.
I-pH 6.0 i-acetate buffer: Chaza u-50 g we-ammonium acetate ku-150 mL wamanzi, lungisa ne-glacial acetic acid ibe i-pH engu-6.0, hlambulula ngamanzi ibe ngu-250 mL, bese uxuba.
Isixazululo esijwayelekile: Lungiselela ingxube ka-2.0 mL yesixazululo se-aluminium Ejwayelekile, u-10 mL we-pH 6.0 buffer ye-acetate, kanye no-98 mL wamanzi.Khipha le ngxube njengoba kuchazwe kusixazululo Sesampula, nciphisa okukhishwe okuhlanganisiwe nge-chloroform kuye kuvolumu, bese uxuba.
Isixazululo esiyisampula: Chaza u-20.0 g we-Anhydrous Citric Acid ku-100 mL wamanzi, bese wengeza u-10 mL we-pH 6.0 acetate buffer.Khipha lesi sixazululo ngezingxenye ezilandelanayo zika-20, 20, kanye no-10 mL wesixazululo esingu-0.5% se-8-hydroxyquinoline ku-chloroform, uhlanganise okukhishwe kwe-chloroform kuflask yevolumu ye-50-mL.Nciphisa okukhishwe okuhlanganisiwe nge-chloroform kuya kuvolumu, bese uxuba.
Isixazululo esingenalutho: Lungiselela ingxube ye-10 mL ye-pH 6.0 i-acetate buffer kanye ne-100 mL yamanzi.Khipha le ngxube njengoba kuchazwe njengesixazululo sesampula, hlambulula okukhishwe okuhlanganisiwe nge-chloroform kuye kuvolumu, bese uxuba.
Izimo ze-Fluorometric
Ubude begagasi besasasa: 392 nm
Ubude begagasi bokuphuma: 518 nm
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile kanye nesixazululo esiyisampula
Thola amandla e-fluorescence Amasampuli kusethi ye-fluorometer njengoba kuqondiswe ngaphansi kwezimo ze-Fluorometric, usebenzisa isixazululo Esingenalutho ukuze usethe ithuluzi libe uziro.
Imibandela yokwamukela: I-fluorescence yesisombululo Sesampuli aweqi isixazululo Esijwayelekile (0.2 ppm).
Ukungcola Okuphilayo
• Inqubo: Umkhawulo we-Oxalic Acid
Isixazululo sesitoko esiyisampula: 200 mg/mL we-Anhydrous Citric Acid emanzini
Isixazululo esiyisampula: Ku-4 mL wesixazululo sesitoko sesampula engeza u-3 mL we-hydrochloric acid ne-1 g ye-zinc eyimbudumbudu, ubilise iminithi elingu-1, bese uvumela ukuthi ime imizuzu emi-2.Dlulisela i-supernatant eshubhuni lokuhlola eliqukethe u-0.25 mL wesisombululo se-phenylhydrazine hydrochloride (1 ku-100), nokushisa kube ukubila.Phola ngokushesha, dlulisela kusilinda esineziqu, bese wengeza umthamo olinganayo we-hydrochloric acid kanye no-0.25 mL wesixazululo se-potassium ferricyanide (1 kokungu-20).Shake, futhi uvumele ume imizuzu engu-30.
Isixazululo esijwayelekile: Lungiselela njengoba kuqondiswe kwisisombululo Sesampuli, ngaphandle kokusebenzisa u-4 mL wesisombululo se-oxalic acid esingu-0.10 mg/mL, okulingana no-0.0714 mg/mL we-anhydrous oxalic acid, esikhundleni sesixazululo sesitoko seSampula.[Qaphela—Lungiselela kanyekanye nesixazululo Sesampula.]
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile kanye nesixazululo esiyisampula
Imibandela yokwamukela: Noma yimuphi umbala ophinki okhiqizwe kusixazululo Sesampuli awuqini kakhulu kunalowo okhiqizwe kusixazululo Esijwayelekile (0.036%).
IZIVIVINYO EZITHILE
• Ukuhlolwa kwe-bacterial Endotoxins Test 85: Izinga lama-endotoxins ebhaktheriya liwukuthi imfuneko efomini lomthamo ofanele we-monograph(s) lapho kusetshenziswa i-Anhydrous Citric Acid ingahlangatshezwana nayo.Lapho ilebula ithi i-Anhydrous Citric Acid kufanele iqhubeke icutshungulwa ngesikhathi kulungiswa amafomu omthamo omjovo, izinga le-endotoxin yebhaktheriya liwukuthi imfuneko efomini lemithamo elifanele lapho kusetshenziswa khona i-Anhydrous Citric Acid ingase isetshenziswe. wahlangana.
• Ukucaca Kwesixazululo
[Qaphela-Isixazululo Sesampula kufanele siqhathaniswe nokumiswa Okujwayelekile A emini kwasemini emi-5 ngemva kokulungiswa kokumiswa Okujwayelekile A. ]
Isixazululo se-Hydrazine sulfate: 10 mg/mL we-hydrazine sulfate emanzini.Vumela ukuma amahora angu-4 kuya kwangu-6 ngaphambi kokusetshenziswa.
Isixazululo se-methenamine: Dlulisa u-2.5 g we-methenamine ku-100-mL we-glass-stoppered flask, engeza u-25.0 mL wamanzi, faka isivalo sengilazi, bese uxuba ukuze uncibilike.
Ukumiswa kwe-opalescent okuyisisekelo: Dlulisa u-25.0 mL wesisombululo se-Hydrazine sulfate ku-25.0 mL wesisombululo se-Methenamine ku-100-mL ye-glass-stopperd ingilazi.Hlanganisa, bese uvumela ukuthi ume amahora angama-24.[Qaphela—Lokhu kumiswa kuzinzile izinyanga ezi-2, inqobo nje uma kugcinwa esitsheni sengilazi esingenasici.Ukumiswa akufanele kuhambisane nengilazi futhi kufanele kuxutshwe kahle ngaphambi kokusetshenziswa.]
Izinga le-Opalescence: Nciphisa u-15.0 mL we-Primary opalescent suspension ngamanzi ukuya ku-1000 mL.[Qaphela—Lokhu kumiswa akufanele kusetshenziswe ngemva kwamahora angu-24 ngemva kokulungiswa.]
Ukumiswa okujwayelekile A: Nciphisa u-5.0 mL we-Opalescence standard ngamanzi ukuya ku-100 mL.
Ukumiswa okujwayelekile B: Nciphisa u-10.0 mL we-Opalescence ejwayelekile ngamanzi ukuya ku-100 mL.
Isixazululo sesampula: 200 mg/mL we-Anhydrous Citric Acid emanzini
Ukuhlaziya
Amasampuli: Ukumiswa okujwayelekile A, Ukumiswa okujwayelekile B, amanzi, kanye nesixazululo sesampula
Dlulisa ingxenye eyanele yesisombululo Sesampuli kushubhu yokuhlola yengilazi engenambala, esobala, engathathi hlangothi enesisekelo esiyisicaba kanye nobubanzi bangaphakathi obungu-15-25 mm ukuze uthole ukujula okungama-40 mm.Ngokufanayo dlulisa izingxenye Zokumiswa Okujwayelekile A, Ukumiswa Okujwayelekile B , namanzi ukuze uhlukanise amashubhu okuhlola afanayo.Qhathanisa isixazululo Sesampuli, ukumiswa okujwayelekile A, ukumiswa okujwayelekile B, namanzi emini ehlukanisiwe, ukubuka kume mpo kungemuva elimnyama (bona i-Spectrophotometry ne-Light-Scattering 851, Ukuqhathanisa Okubonakalayo).[Qaphela—Ukusatshalaliswa kokukhanya kumelwe kube ngendlela yokuthi ukumiswa okujwayelekile A kukwazi ukuhlukaniswa kalula emanzini, nokuthi ukumiswa okujwayelekile okujwayelekile B kungahlukaniswa kalula nokumiswa Okujwayelekile A. ]
Imibandela yokwamukela: Isixazululo Sesampula sibonisa ukucaca okufanayo nalokhu kwamanzi.
• Umbala Wesixazululo
Isixazululo sesitoko esijwayelekile A: I-Ferric chloride CS, i-cobaltous chloride CS, ne-dilute hydrochloric acid (10 g/L) (2.4:0.6:7.0)
Isixazululo sesitoko esijwayelekile B: I-Ferric chloride CS, i-cobaltous chloride CS, i-cupric sulfate CS, ne-dilute hydrochloric acid (10 g/L) (2.4:1.0:0.4:6.2)
Isixazululo sesitoko esijwayelekile C: I-Ferric chloride CS, i-cobaltous chloride CS, ne-cupric sulfate CS (9.6:0.2:0.2)
[Qaphela-Lungiselela izixazululo Ezijwayelekile ngokushesha ngaphambi kokusetshenziswa.]
Isixazululo esijwayelekile A: Nciphisa u-2.5 mL wesixazululo sesitoko Esijwayelekile A nge-dilute hydrochloric acid (10 g/L) ukuya ku-100 mL.
Isixazululo esijwayelekile B: Nciphisa u-2.5 mL wesixazululo sesitoko Esijwayelekile B nge-dilute hydrochloric acid ukuya ku-(10 g/L) ku-100 mL.
Isixazululo esijwayelekile C: Nciphisa u-0.75 mL wesixazululo sesitoko Esijwayelekile C nge-dilute hydrochloric acid (10 g/L) ukuya ku-100 mL.
Isixazululo sesampula: Sebenzisa Isampula yesixazululo esilungiselelwe njengoba kuqondiswe ekuhlolweni kokucaca kwesixazululo.
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile A, Isixazululo esijwayelekile B, Isixazululo esijwayelekile C, amanzi, kanye nesixazululo esiyisampula
Dlulisa ingxenye eyanele yesisombululo Sesampuli kushubhu yokuhlola yengilazi engenambala, esobala, engathathi hlangothi enesisekelo esiyisicaba kanye nobubanzi bangaphakathi obungu-15-25 mm ukuze uthole ukujula okungama-40 mm.Ngokufanayo dlulisa izingxenye zesixazululo Esijwayelekile A, Isixazululo Esijwayelekile B, Isixazululo Esijwayelekile C, namanzi ukuze uhlukanise amashubhu okuhlola afanayo.Qhathanisa isixazululo Sesampula, Isixazululo Esijwayelekile A, Isixazululo Esijwayelekile B, Isixazululo Esijwayelekile C, namanzi emini ehlukanisiwe, ubuka uqonde kungemuva elimhlophe (bheka i-Spectrophotometry ne-Light-Scattering 851, Ukuqhathanisa Okubonakalayo).
Indlela yokwamukela: Isixazululo Sesampula asinawo umbala ogqamile kunesixazululo Esijwayelekile A, B, noma C, noma amanzi.
• Izinto ezitholakala kalula ngeCarbonizable
Isampula: 1.0 g ye-Anhydrous Citric Acid eyimpuphu
Ukuhlaziya: Dlulisela Isampula kushubhu yokuhlola engu-22- × 175-mm ehlanjululwe ngaphambilini ngo-10 mL we-sulfuric acid futhi ivunyelwe ukudonsa imizuzu eyi-10.Engeza u-10 mL we-sulfuric acid, gudluza kuze kuphele isixazululo, bese ucwilisa emanzini okugeza ku-90 ± 1 imizuzu engama-60 ± 0.5, ugcine izinga le-asidi ngaphansi kwezinga lamanzi phakathi naso sonke isikhathi.Pholisa ishubhu emanzini agobhozayo, bese udlulisela i-asidi eshubhuni lokuqhathanisa umbala.
Imibandela yokwamukela: Umbala we-asidi awumnyama kunalowo wevolumu efanayo ye-Matching Fluid K (bona Umbala kanye ne-Achromicity 631) eshubhuni elifanayo, amashubhu abhekwa ngokuqondile ngokumelene nengemuva elimhlophe.
• Ukuhlolwa Kokuzala 71: Lapho ilebula ithi i-Anhydrous Citric Acid iyinyumba, ihlangabezana nezimfuneko zokuhlolwa kwe-Sterility Test 71 efomini lomthamo ofanele lapho kusetshenziswa khona i-Anhydrous Citric Acid.
• Ukunqunywa kwamanzi, Indlela I 921: NMT 1.0%
IZIDINGO EZENGEZIWE
• Ukupakisha Nokugcina: Londoloza ezitsheni eziqinile.Azikho izidingo zesitoreji ezishiwo.
• Ukulebula: Lapho kuhloswe khona ukuthi kusetshenziswe izixazululo ze-dialysis, kubhalwe kanjalo.Lapho i-Anhydrous Citric Acid kufanele iqhubeke nokucutshungulwa ngesikhathi sokulungiswa kwamafomu okujovwa ukuze kuqinisekiswe amazinga amukelekayo e-endotoxin yebhaktheriya, ibhalwe kanjalo.Lapho i-Anhydrous Citric Acid iyinyumba, ibhalwe kanjalo.
• I-USP Reference Standards 11
USP Citric Acid RS Chofoza ukuze Ubuke Isakhiwo
USP Endotoxin RS