I-β-Cyclodextrin (β-CD) CAS 7585-39-9 Assay 98.0%~102.0% Factory

Ukuzungezisa okuqondile
Thatha lo mkhiqizo, ukale kahle, ungeze amanzi ukuze uwancibilikise futhi uwahlambulule ngobuningi ube yisixazululo esiqukethe cishe u-10 mg nge-1 ml ngayinye, futhi uwunqume ngokomthetho (Umthetho ojwayelekile 0621), ukuzungezisa okuqondile kwakungu-159 ° kuya ku-164 °.
Ukuxilongwa okuhlukile
Thatha cishe u-0.2g walo mkhiqizo, engeza u-2ml wesisombululo sokuhlola i-Iodine, usishise emanzini okugeza ukuze uyincibilikise, futhi uyeke uphole ukuze ukhiqize imvula ephuzi ngokunsundu.
Kuchromatogram erekhodwe ngaphansi kwento yokunquma okuqukethwe, isikhathi sokugcinwa kwenani eliphakeme lesixazululo sokuhlola kufanele sihambisane nesikhathi sokugcinwa kwenani eliphakeme lesixazululo sokulawula.6
I-spectrum yokumuncwa kwe-infrared yalo mkhiqizo kufanele ihambisane naleyo yomkhiqizo oyinkomba (Umthetho ojwayelekile 0402).
Ukungabi bikho kokungcola
Thatha lo mkhiqizo cishe u-1 g, isisindo esinembile, engeza amanzi angu-100ml ukuze uncibilike, ngokuya ngokunqunywa kwe-UV-visible spectrophotometry (Umthetho ojwayelekile 0401), ukumunca ebangeni le-wavelength elingu-230~350nm akumele kudlule u-0.10, ukumuncwa ebangeni le-wavelength kokungu-350~750nm akufanele kudlule u-0.05.
pH
thatha u-0.20g walo mkhiqizo, engeza u-20ml wamanzi ukuze uncibilike, engeza u-0.2ml wesisombululo se-potassium chloride esigcwele, futhi unqume ngokomthetho (Umthetho ojwayelekile 0631), inani le-pH kufanele libe ngu-5.0~8.0.
Ukucaca nombala wesixazululo
Thatha lo mkhiqizo 0. 50g, engeza u-50ml wamanzi ukuze uhlakaze, hlola ngokomthetho (Umthetho ojwayelekile 0901 kanye nomthetho jikelele we-0902), isisombululo kufanele sicace futhi singabi mbala;Uma kune-turbidity, qhathanisa ne-No. 2 Turbidity standard solution (Umthetho ojwayelekile 0902, indlela yokuqala), hhayi ukugxila kakhulu.
I-Chloride
Thatha lo mkhiqizo ongu-0.39G, uhlolwe ngokomthetho (Umthetho ojwayelekile 0801), kanye nesisombululo se-sodium chloride engu-7.0ml akumele sigxiliswe kakhulu kunesixazululo sokulawula (0.018%).
Ukunciphisa ushukela
Thatha lo mkhiqizo 1.0 g, ukunemba enesisindo, engeza amanzi 25ml ukuncibilika, Faka alkaline yethusi tartrate test solution 40tnl, kancane ubilise imizuzu 3, beka ekamelweni lokushisa ubusuku bonke, filter nge 4 # vertical melting funnel, precipitate wageza ngamanzi afudumele. kuze kube yilapho isisombululo sokugeza singathathi hlangothi.Isixazululo sokuhlunga nesokugeza siyalahlwa, kuyilapho kushisa ngesisombululo se-titration se-potassium permanganate (0 .0M ol/L).Ngokomkhiqizo owomile, ukusetshenziswa kwe-potassium permanganate titrant (0.02 Mol/l) ku-L g akumele kudlule u-3.2ml(1.0%).
I-Cyclohexane
Thatha cishe u-0.2g walo mkhiqizo, ukulinganisa okunembile, ebhodleleni elingenalutho eliphezulu, engeza isisombululo esijwayelekile sangaphakathi (thatha inani elifanele le-dichlorethylene, engeza isixazululo se-dimethyl sulfoxide esingu-20% ukuze wenze ikhambi eliqukethe cishe u-0.04 ku-lm l ngayinye, (Ilungile ) 10.0ml, njengesixazululo sokuhlola; Okunye ukunemba kwesisindo se-cyclohexane, kanye nesixazululo esijwayelekile sangaphakathi sokwenza cishe ilitha elingu-1/l eliqukethe i-cyclohexane 0.078mg yesixazululo, kala u-10.0ml ebhodleleni elingenalutho eliphezulu njengesixazululo sokulawula. ukunqunywa kwe-solvent eyinsalela (Umthetho ojwayelekile 0861), ikholomu ye-capillary ene-100% dimethylpolysiloxane njengoketshezi olunganyakazi isetshenziswa njengekholomu ye-chromatographic; Izinga lokushisa lekholomu lingu-90 ℃; izinga lokushisa elingenayo lingu-200 ℃; izinga lokushisa lomtshina lingu-250 ℃; ibhodlela le-Headspace izinga lokushisa lokulinganisa lalingu-70℃ futhi isikhathi sokulinganisa sasiyimizuzu engu-20. Thatha isisombululo sereferensi usifake ku-headspace, idigri yokuhlukanisa phakathi kweziqongo zengxenye ngayinye kufanele ihambisane nezinhlinzeko.Isixazululo sokuhlola kanye nesixazululo sereferensi kufakwa ngokulandelana ku-headspace, i-chromatogram iyarekhodwa, futhi indawo ephakemeyo ibalwa ngokuvumelana nendlela evamile yangaphakathi.
Ukulahlekelwa ekomisweni
Thatha lo mkhiqizo, womile kuya kwesisindo esingaguquki ku-105 ℃, ukuncipha kwesisindo akumele kudlule u-14.0% (Umthetho ojwayelekile 0831).
Izinsalela zokubasa
I-1.0 g yalo mkhiqizo izothathwa ukuze ihlolwe ngokuvumelana nomthetho (Umthetho Ojwayelekile 0841), futhi okusele okusele akumele kudlule.
Izinsimbi ezinzima
Izinsalela ezishiywe ngaphansi kwento yokuthatha insalela yokubasa ngeke ziqukathe izingxenye ezingaphezu kweziyi-10 esigidini sensimbi esindayo lapho ihlolwa ngokomthetho (Izimiso Ezijwayelekile 0821, Law II).
Umkhawulo we-Microbial
Lo mkhiqizo uzothathwa futhi uhlolwe ngokomthetho (Izimiso Ezijwayelekile 1105 kanye ne-1106).Inani eliphelele lamabhaktheriya e-aerobic nge-lg yomkhiqizo wokuhlola akumele lidlule i-100cfu, inani eliphelele lesikhunta nemvubelo akufanele lidlule i-100cfu, i-E. Coli akufanele ibonwe.
Ukuzimisela kokuqukethwe
Ikalwa ngokusebenza okuphezulu kwe-chromatography ye-liquid (Okujwayelekile 0512).
Izimo zeChromatographic kanye nokuhlolwa kokufaneleka kwesistimu Ijeli ye-silica ehlanganisiwe ye-alkyl eyishumi nesishiyagalombili yasetshenziswa njengesigcwalisi;I-Water-methanol (85 : 15) isetshenziswe njengesigaba esihambayo;Futhi ukulinganisa kwenziwa ngomtshina wenkomba we-refractive ohlukile.Inombolo yepuleti yetiyori ayikho ngaphansi kuka-1500 ebalwa njengenani eliphakeme le-betal cyclodextrin.
indlela yokunquma: thatha cishe u-50mg walo mkhiqizo, ukale ngokunembile, uwubeke ku-flask yokulinganisa engu-10ml, engeza inani elifanele lamanzi ukuze uhlakaze futhi uhlambulule esikalini, unyakazise kahle, futhi uyisebenzise njengesixazululo sokuhlola, 10/xl. yafakwa ku-chromatograph ewuketshezi ngokunembile, futhi i-chromatogram yarekhodwa.Enye i-50mg yereferensi ye-betaloc cyclodextrin ikalwe ngokunembile, futhi indlela efanayo yasetshenziswa ukuze kunqunywe.Ngokwendlela evamile yangaphandle yokubala indawo ephakemeyo, okungukuthi.