I-Fluorescamine CAS 38183-12-9 Ubumsulwa >98.0% (HPLC)
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Igama Lekhemikhali | I-Fluorescamine |
Omqondofana | 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione |
Inombolo ye-CAS | 38183-12-9 |
Inombolo yeCAT | I-RF-PI2189 |
Isimo Sesitoko | Esitokweni, Isikali Sokukhiqiza Sifinyelela Kumathani |
I-Molecular Formula | I-C17H10O4 |
Isisindo samangqamuzana | 278.26 |
Ukuzinza | I-Hygroscopic |
Gcina Ngaphansi Kwegesi Ye-inert | Gcina Ngaphansi Kwegesi Ye-inert |
Ukuncibilika | Incibilika ku-acetone, i-Ethanol, i-Chloroform, i-Dimethylsulfoxide ne-Acetonitrile.Incibilika Kancane Emanzini |
I-Spectral Properties | Injabulo (nm): 382, Emission (nm): 480, Absorbance (nm): 237 |
Ibhrendi | I-Ruifu Chemical |
Into | Imininingwane |
Ukubukeka | Impushana Ephuzi Ekhanyayo |
Ukuhlanzeka / Indlela Yokuhlaziya | >98.0% (HPLC) |
I-Melting Point | 153.0 ~ 157.00℃ |
Amanzi (nguKarl Fischer) | <0.50% |
Ukungcola Okuphelele | <2.00% |
Ikhabhoni ngokuhlaziywa kwe-Elemental | 72.88~73.88% (Amanzi) |
I-Molar Extinction Coefficient | I-Diethyl Ether, 233.0 kuya ku-235.0nm |
Ubunikazi nge-NMR (CDCl3) | Kuyavumelana |
Ukuncibilika ku-acetone | Ayinambala ukuya kokuphuzi, Icacile, 50.00 mg/ml Pass |
Izinga Lokuhlola | I-Enterprise Standard |
Iphakheji: Ibhodlela, isikhwama se-Aluminium foil, 25kg/Cardboard Drum, noma ngokwemfuneko yekhasimende
Isimo Sesitoreji:Gcina ezitsheni ezivaliwe endaweni epholile neyomile;Ukungenisa umoya;2~8℃;Vikela ekukhanyeni nasemanzini;Ukuvikelwa kwe-nitrogen kungandisa impilo yeshalofu
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I-Fluorescamine (i-CAS: 38183-12-9) i-reagent engeyona i-fluorescent esabela kalula ngaphansi kwezimo ezithambile nama-amine ayinhloko kuma-amino acid nama-peptide ukuze enze izinhlanganisela ezizinzile, ezikhanyayo kakhulu.Ukusabela kwenzeka ngaphansi kwezimo ezithambile.Ingemuva eliphansi ngenxa ye-hydrolysis.I-reagent yokuhlaziya.Ukutholwa kwe-fluorescent yama-amino acid, ama-peptide namaprotheni.I-Fluorescamine idlala indima ebalulekile njenge-reagent yokuthola ama-amine, ama-peptide namaprotheni.Ibuye isetshenziswe njenge-substrate yokulinganisa umsebenzi we-protease.Isetshenziswa ekutholeni nasekulinganiseni inani lama-aminopenicillin ayinsalela nama-sulfonamide oju yi-HPLC.Ithola ukusetshenziswa ekunqumeni kwe-lisinopril ku-plasma yomuntu kanye nomchamo.Ivimba ngokwanele i-amino termini esanda kukhiqizwa ekuhlaziyweni kokulandelana kwamaprotheni.I-Fluorescamine iyi-reagent edumile ye-amine-fluorogenic yokunquma ukugxila kwamaprotheni kuzixazululo nasemajeli.Ibuye isetshenziselwe ukuhlaziya i-low molecular weight amines nge-TLC, HPLC kanye ne-capillary electrophoresis.I-Fluorescamine ayiyona i-fluorescent kodwa isabela kalula nama-aliphatic amines ayinhloko, okuhlanganisa nalawo aku-peptide namaprotheni, ukuze kukhiqizwe i-fluorescent eluhlaza okwesibhakabhaka ngokunamandla engajatshuliswa ukukhanya kwe-UV.I-amine adduct inokumunca okuphezulu ku-385 nm kanye ne-fluorescence esiphezulu ku-486 nm.