I-Flurbiprofen CAS 5104-49-4 Assay 99.0~100.5% (HPLC) Factory

I-Flurbiprofen
I-C15H13FO2 244.27
(±)-2-(2-Fluoro-4-biphenylyl) i-propionic acid [5104-49-4].
I-Flurbiprofen iqukethe amaphesenti angekho ngaphansi kwamaphesenti angu-99.0 futhi angabi ngaphezu kwamaphesenti angu-100.5 we-C15H13FO2, abalwe ngesisekelo esomisiwe.
Ukupakisha nokugcina- Londoloza ezitsheni eziqinile.
Amazinga ereferensi we-USP <11>-
USP Flurbiprofen RS
I-USP Flurbiprofen Inhlanganisela Ehlobene A RS
2-(4-Biphenylyl)propionic acid.
C15H14O2 226.28
Ukuhlonza-
A: I-Infrared Absorption <197K>.
B:Ukumuncwa kwe-Ultraviolet 197U-
Isixazululo: 10 µg ngemL.
Okuphakathi: 0.1 N sodium hydroxide.
Ubukhulu be-Absorbance ku-247 nm bungaba ngu-0.8.
Ibanga lokuncibilika <741>: phakathi kuka-114 no-117.
Ukulahlekelwa lapho ukoma <731>-Yomise ku-vacuum ku-60 kuya esisindweni esingaguquki: ilahlekelwa ngaphezu kuka-0.5% wesisindo sayo.
Izinsalela ekushiseni <281>: hhayi ngaphezu kuka-0.1%.
Izinsimbi ezinzima, Indlela II <231>: 0.001%.
Izinhlanganisela ezihlobene-
I-Diluent-Lungisa ingxube yamanzi ne-acetonitrile (11:9).
Isigaba seselula-Lungiselela ingxube ehlungiwe nekhishiwe amanzi, i-acetonitrile, ne-glacial acetic acid (12:7:1).Yenza izinguquko uma kudingeka (bona Ukufaneleka Kwesistimu ngaphansi kwe-Chromatography <621>).
Isixazululo sesitoko esijwayelekile- Chaza isamba esikalwe ngokunembile se-USP Flurbiprofen Related Compound A RS ku-Diluent ukuze uthole isixazululo esinokugxila okungaba ngu-50 µg ngemL ngayinye.
Isixazululo sokufaneleka kwesistimu-Ipayipi elingu-2.0 mL lesisombululo sesitoko Esijwayelekile ku-10-mL ye-volumetric flask, engeza cishe u-20 mg we-USP Flurbiprofen RS, hlambulula nge-Diluent kuya kuvolumu, bese uxuba.
Isixazululo esijwayelekile-Dlulisa u-2.0 mL wesisombululo sesitoko Esijwayelekile ku-10.0-mL ye-volumetric flask, hlambulula nge-Diluent kuya kuvolumu, bese uxuba.
Isixazululo sokuhlola-Lungiselela isixazululo se-Flurbiprofen ku-Diluent equkethe u-2.0 mg ngemL ngayinye.
Isistimu ye-Chromatographic (bona i-Chromatography <621>)-I-chromatograph ewuketshezi ifakwe umtshina ongu-254-nm kanye nekholomu engu-3.9-mm × 15-cm equkethe 4-µm ukupakisha L1.Izinga lokugeleza licishe libe ngu-1 mL ngomzuzu.I-Chromatograph isixazululo sokufaneleka kweSistimu, bese urekhoda izimpendulo eziphezulu njengoba kuqondiswe kuNqubo: izikhathi zokugcinwa okuhlobene zicishe zibe ngu-0.9 kunhlanganisela A ehlobene ne-Flurbiprofen kanye no-1.0 nge-flurbiprofen;futhi ukuchezuka okujwayelekile okuhlobene kwemijovo eyimpinda akukho ngaphezu kuka-1.0%.
Inqubo-Ngokuhlukile jova amavolumu alinganayo (cishe u-20 µL) wesisombululo Esijwayelekile kanye nesixazululo sokuhlola ku-chromatograph, rekhoda ama-chromatogram, futhi ulinganise izindawo zeziqongo ezinkulu.Bala iphesenti le-flurbiprofen ehlobene nenhlanganisela A engxenyeni ye-Flurbiprofen ethathwe ifomula:
100(CS / CU)(rU / rS)
lapho i-CS iwukugxilisa ingqondo, ku-µg ngemL ngayinye, ye-USP Flurbiprofen Ehlobene Inhlanganisela A RS kusixazululo Esijwayelekile;I-CU ukugxila, ku-µg ngemL ngayinye, ye-Flurbiprofen kusixazululo sokuhlola;kanye ne-rU kanye ne-rS yizimpendulo eziphezulu zenhlanganisela A ehlobene ne-flurbiprofen etholwe kusixazululo sokuhlola kanye nesixazululo esijwayelekile, ngokulandelana: akutholakali ngaphezu kuka-0.5% wenhlanganisela A ehlobene ne-flurbiprofen.Bala iphesenti lokungcola ngakunye engxenyeni ye-Flurbiprofen ethathwe ifomula:
100(ri/rs)
lapho i-ri iyimpendulo ephakeme kakhulu yokungcola ngakunye okutholakala kusixazululo sokuhlola;futhi u-rs uyisamba sezimpendulo zazo zonke iziqongo ezitholwe kusixazululo sokuhlola: isamba sakho konke ukungcola asikho ngaphezu kuka-1.0%.
I-Assay-Dissolve cishe u-0.5 g we-Flurbiprofen, enesisindo esinembile, ku-100 mL wotshwala, owake waba yingxenye ye-0.1 N sodium hydroxide VS endaweni yokugcina ye-phenolphthalein, engeza i-phenolphthalein TS, futhi i-titrate nge-0.1 Nsodium hydroxide VS ekubukekeni okuncane kokuvela kokuqala. umbala ophinki ophikelelayo okungenani imizuzwana engama-30.I-mL ngayinye ye-0.1 N sodium hydroxide ilingana no-24.43 mg we-C15H13FO2.