I-H-Glu(OEt)-OEt·HCl CAS 1118-89-4 L-Glutamic Acid Diethyl Ester Hydrochloride Purity >99.0% (HPLC) Factory

I. Izinqubo zokuhlola
1. Iphuzu lokuncibilika
Ithuluzi lokuncibilika kwedijithali liyasetshenziswa
Isinyathelo sesi-2: Okuqukethwe
2.1 Ama-reagents
Isinyathelo 1: I-Formic acid
2. Glacial acetic acid
3. Isixazululo esijwayelekile se-Perchloric acid (C=0.1000mol/l)
4. Uketshezi lwenkomba ye-Crystal Violet: 5g/l.
2.2 Izinsimbi nezinto zokusebenza
1. I-beaker 150ml
2. I-Microburet: 10ml
2. 3 Izinyathelo zokuhlaziya
2. 3. Ngikala ngokunembile mayelana ne-300mg yesampula eyomisiwe ngaphambili ku-105℃ nge-3h, ngiyibeke ku-bethel engu-150ml, engeza u-3ml we-formic acid, engeza u-50ml we-acetic acid, engeza amaconsi angu-2 esixazululo sokuhlola i-crystal violet ( TS-74), kanye ne-titrate ngo-0.1mol/L i-perchloric acid kuze kube indawo yokugcina eluhlaza noma kuze kube yilapho okuluhlaza okwesibhakabhaka kunyamalala ngokuphelele.
2. 3. 2 Indlela yokubala
U-X ulingana no-C (V1-V2) izikhathi ezingu-0.2042 / M izikhathi ezingu-100
I-C ingxube yesisombululo se-perchloric acid esijwayelekile se-titration, esivezwa ngama-moles ilitha ngalinye (i-mol/l).
I-V1 ivolumu esetshenzisiwe yesisombululo esigxilisiwe se-perchloric acid ye-titration kusampula ye-titration, evezwa ngo-ml (ml).
Ivolumu edliwe yesisombululo se-perchloric acid esijwayelekile sokulinganisa esivivinyweni esingenalutho se-V2, esivezwe ngo-ml (ml)
0.2042 kanye no-1.00ml i-perchloric acid ejwayelekile yesigaba soketshezi esigxilisiwe lapho ikhwalithi yalo mkhiqizo ivezwa ngo-G
M Izinga lesampula, elivezwe ngamagremu (g).
2. 4 Ukulungiswa
Uma umehluko wezinga lokushisa legumbi phakathi kwesisombululo sokuhlola i-titration nesisombululo esijwayelekile se-perchloric acid sokulinganisa udlula u-10℃, kufanele silinganiswe kabusha;Uma kungenjalo, i-concentration C1 yesisombululo esijwayelekile se-perchloric acid izolungiswa ngokuya ngokuhlolwa (2).
C2= C1/1+0.0011 (t1-t2)
I-1+0.0011 iyi-coefficient enwetshiwe ye-glacial acetic acid
Iyunithi yezinga lokushisa legumbi lokulinganiswa kwe-T2 kwe-perchloric acid nguCelsius (° C).
Iyunithi yezinga lokushisa legumbi lapho isampula ifakwe titrated nguCelsius (° C).
I-C1 t2℃ i-perchloric acid standard solution concentration, kuma-moles ilitha ngalinye (MOL/L)
3. Eyomile futhi engenasisindo
3.1 Izinsimbi:
I-ovini yokomisa i-Thermostatic, ibhalansi engu-1/10,000.
3.2 Inqubo:
Thatha amagremu angu-1-2 wamasampula (okunembe ku-0.5mg) uwafake ebhodleleni lesisindo esilomiswe ngaphambili futhi esikalini.Amasampula azoba nokujiya okufanayo phansi kwebhodlela lokukala.Ngokuqondene namasampuli, azomiswa kuhhavini we-thermoelectric (105±5℃) ukuze abe nesisindo esingaguquki.
3.3 Ukubala
U-X ungu-m susa m1 uhlukaniswa ngo-m susa m2 izikhathi ezingu-100
Kuphi: Umswakama ongu-X %
m1 -- Isisindo sesampula esomile kanye nebhodlela lokulinganisa g
m2 - Kala ubukhulu bebhodlela g
m- Isisindo sesampula g
3.4 Amaphuzu Okunakwa
1. Amaphuzu okunakwa: 1. Amasampula afanayo kumele enziwe ukuze omiswe futhi angabi nesisindo.Isampula eyodwa inemiphumela emibili, futhi iphutha phakathi kwemiphumela emibili alikho ngaphezu kuka-0.5%.
2. Ukukala kufanele kucophelele, kunembile ku-0.0001.
4 ukuzungeza
4.1 Izimiso
Ukukhanya okukhazimulayo kwendiza okudlula oketshezini noma isixazululo senhlanganisela equkethe umsebenzi othile wokubona (okungukuthi, into esebenza ngokubonakalayo) kungabangela ukuzungezisa kwe-optical, kubangele indiza yokukhanya okumhlophe ukuthi ijikele kwesokunxele noma kwesokudla.Inombolo yamadigri okuzungezisa ibizwa ngokuthi i-optical rotation.Ezingeni elithile lokushisa (ngokuvamile elivezwa ngu-t, lingaba ngu-20℃ noma 25℃) ubude beza beza bokukhanya (ukukhanya kwesodium ephuzi no-D, ubude be-wavelength λ 589.3nm) ukukhanya okuhlukanisiwe nge-milliliter ngayinye equkethe i-1g optical activity, ukujiya okungu-1dm. (okungukuthi, 10cm) isixazululo lapho ukuzungezisa kubizwa ngokuthi ukuzungezisa okukhethekile.
4.2 Izidingo
Umugqa we-D we-sodium spectrum (589. 3 nm) wasetshenziswa ukukala ubude beshubhu obungu-1dm futhi izinga lokushisa lalingu-20℃.
4.3 Izinsimbi
4.3.1 I-autotropy yedijithali (ukunemba ±0.001°)
4.3.2 Ibhalansi ye-elekthronikhi (ukunemba 0.0001g).
4.4 Indlela Yokusebenza
Thatha i-3g yalo mkhiqizo, enembile ku-0.0001g, uyibeke ebhodleleni le-volumetric engu-100ml, engeza i-ethanol ukuze uhlakaze futhi uyihlambulule esikalini, unyakazise kahle.Hlanza ishubhu lokulinganisa ngenani elincane loketshezi izikhathi ezimbalwa, bese ujova uketshezi kancane kancane eshubhuni yokulinganisa (ungawenzi amabhamuza avele), beka ishubhu lokulinganisa ku-gyroscope ukuze libonwe, futhi ufunde ukufundwa kokuzungezisa ukubona Izikhathi ezi-3 ngokuhambisana.
4.5 Ukubala
Ukuzungezisa okuthile kwe-L-glutamate Diethyl carbonate kuya ku-sodium spectrum D-line ku-20℃ [Alpha]D20 kubalwe ngale ndlela elandelayo:
[ɑ] D20 = alpha/m
Lapho: α -- isilinganiso senani lokufundwa kokuzungeziswa kwe-cubic;
m -- ikhwalithi yesampula.
4.6 Umehluko ovumelekile: Umehluko ophelele wesampula efanayo ngeke udlule u-0.02% uma ukalwa kabili.
4.7 Izinyathelo zokuqapha
4.7.1 I-solvent kufanele isetshenziswe njengokulinganisa okungenalutho ngaphambi kwesilinganiso ngasinye: Ngemva kokulinganisa, ukulinganisa kufanele kwenziwe futhi ukuze kunqunywe ukuthi iphoyinti elinguziro liyashintsha yini ngesikhathi sokulinganisa.Uma kukhona izinguquko, ukuzungezisa kokubona kwesampula kufanele kunqunywe futhi.
4.7.2 Isampula kufanele icaciswe ngemva kokuchithwa kanye nokuhlanjululwa;kungenjalo, kufanele ihlungwe.
Isinyathelo sesi-5 Ukubukeka
Umkhiqizo uzoba Impushana encane emhlophe ukuya kokumhlophe