L-4-Nitrophenylalanine Methyl Ester HCl CAS 17193-40-7 H-Phe(4-NO2)-OMe·HCl Assay >98.5% (HPLC) Factory

Indlela Yokuhlaziya ye-L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7)
Incazelo: Faka cishe u-2 g wesampula esitsheni esihlanzekile nesomile bese ubheka ukubukeka kwesampula.Ukubika: Bika umbono
I-Identificaiton: Qhathanisa isikhathi sokugcinwa kwenani eliphakeme le-chromatogram yokulungiselelwa kokuhlolwa nesikhathi sokugcinwa kwenani eliphakeme le-chromatogram ye-praparation yokufaneleka kwesistimu njengoba kutholwe ekuhlanzekeni kwe-chromatographic yi-HPLC.
Ukulahlekelwa Ekumisweni: Yomisa ibhodlela elinesisindo esingajulile, elingashoni ku-105±2℃ imizuzu engama-30.Livumele liphole lifike kuzinga lokushisa elilingana negumbi kumshini wokukhipha amanzi.Kala ngokunembile ibhodlela lokukala ngesivalo bese ubhala isisindo (W1).Dlulisela inani elinembile lesisindo esingu-1 kuye ku-2g ebhodleleni lesisindo.Ngokunyakazisa okumnene, eceleni, sabalalisa isifanekiso sokuhlola ngokulinganayo ngangokunokwenzeka ekujuleni okungaba ngu-5 mm.Kala ibhodlela lesisindo elilayishiwe bese urekhoda isisindo (W2).Beka ibhodlela elilayishiwe kuhhavini owomisa, ukhiphe isivalo bese usishiya futhi ekamelweni.Yomisa isampula yokuhlola ku-105±2℃ amahora angu-3.Lapho uvula igumbi, vala ibhodlela ngokushesha, futhi ulivumele ukuthi lifike ekamelweni lokushisa ku-desikacator.Kala ngokunembile ibhodlela lokukala ngesivalo bese uphinda isisindo (W3).Bala ukulahlekelwa kwephesenti ekomisweni kwesampula ngokusebenzisa ifomula elandelayo:
Ukubala Ukulahlekelwa ekomisweni (% w/w)= (W2-W3) ÷ (W2-W1) x 100
Okuqukethwe kwe-Enantiomer nge-HPLC:
Amakhemikhali nama-reagents:
Ibanga le-n-Hexane AR
Ibanga le-Methanol HPLC
I-Isopropyl Alcohol HPLC ibanga
Ibanga le-Diethylamine AR
Amazinga: L-4-Nitrophenylalanine Methyl Ester HCl ejwayelekile;L-4-Nitrophenylalanine Methyl Ester HCl enantiomer ejwayelekile
Isigaba Esihambayo: Lungiselela ingxube ye-homogeneous ye-n-Hexane, Isopropyl Alcohol, Methanol ne-Diethylamine (65:20:15:0.1 v/v/v/v)
Ukulungiselela Okungenalutho: Dlulisa u-2.5ml we-Methanol ku-10-ml ye-volumetric flask, nciphisa kuvolumu ngesigaba seselula bese uxuba.
Ukulungiswa kokufaneleka kwesistimu:
Kala ngokunembile bese udlulisa cishe u-5 mg ngayinye ye-L-4-Nitrophenylalanine Methyl Ester HCl ejwayelekile kanye ne-L-4-Nitrophenylalanine Methyl Ester HCl indinganiso ye-enantiomer ku-10-ml ye-volumetric flask, ncibilika cishe ku-2.5ml we-methanol, nciphisa ivolumu ngeselula isigaba bese uxuba.
Ukulungiselela ukuhlolwa:
Kala ngokunembile futhi udlulise cishe u-5 mg wesampula kufulaski wevolumu ye-10-mol, ncibilikisa cishe ku-2.5ml we-methanol, nciphisa kuvolumu ngesigaba seselula bese uxuba.
Amapharamitha wezinsimbi:
Insimbi: I-HPLC ifakwe umjovo, Iphampu, umtshina we-UV kanye neRekhoda
Ikholomu: ChiralPak-IC (4.6mm x 250mm) 5 μ noma okulinganayo
Izinga lokugeleza: 1.0 ml/ngomzuzu
Ubude begagasi: 260nm
Umthamo womjovo: 10 μL
Izinga lokushisa lekholamu yekholomu: 25℃
Isikhathi sokugijima: imizuzu engu-20
Inqubo
Jova izixazululo njengoba kushiwo ngokulandelana okulandelayo.Rekhoda ama-chromatogram futhi uqinisekise imibandela:
Incazelo Yenombolo Yemijovo Yemibandela Yokujova
Ukulungiselela Okungenalutho 1 Akukho ukuphakama okuphazamisayo okufanele kubonwe ngesikhathi sokugcinwa kwe-L-4-Nitrophenylalanine Methyl Ester HCl noma i-enantiomer.
Ukulungiswa kokufaneleka kwesistimu 1 Ukulungiswa phakathi kwe-L-4-Nitrophenylalanine Methyl Ester HCl kanye nenani eliphakeme le-enantiomer akufanele libe ngaphansi kuka-2.0
Ukulungiselela ukuhlolwa 1 Sebenzisa i-chromatogram yokuhlola ukuze unqume okuqukethwe kwe-enantiomer kusampula.
Izikhathi Zokugcina Ezihlobene:
I-L-4-Nitrophenylalanine Methyl Ester HCl 1.0
I-Enantiomer Mayelana ne-1.11
Isikhathi sokugcinwa kwe-L-4-Nitrophenylalanine Methyl Ester HCl cishe imizuzu eyi-9.8.
Ukubala: Kuchromatogram etholwe ekulungiseleleni ukuhlolwa, shaya indiva zonke iziqongo lindela i-L-4-Nitrophenylalanine Methyl Ester HCl ne-Enantiomer.Bika okuqukethwe kwe-enantiomer kusampula ngendlela yokujwayela yendawo.
I-Chromatogrpahic Purity and Ukungcola nge-HPLC:
Amakhemikhali nama-reagents:
I-Diammonium phosphate AR ibanga
I-Phosphoric acid AR ibanga
Ibanga le-Methanol HPLC
Amanzi Ahlanzekile
Amazinga
I-L-4-Nitrophenylalanine Methyl Ester HCl ejwayelekile
4-Nitro-L-Phenylalanine monohydrate standard
Isigaba seselula-A
Chaza u-6.6 g we-Diammonium phosphate ku-1000ml wamanzi ahlanzekile.Lungisa i-pH yalesi sixazululo ibe ngu-6.0 nge-Phosphoric acid.Isihlungi kanye ne-degas.Ngaphambi kokukala i-pH yesisombululo, linganisa imitha ye-pH ngesisombululo sebhafa esijwayelekile i-pH 4.01 KANYE no-7.00.
I-Mobile Phase-B
Ibanga le-Methanol HPLC
Ukulungiselela Ukufaneleka Kwesistimu
Kala ngokunembile futhi udlulise cishe u-25 mg ngayinye ye-L-4-Nitrophenylalanine Methyl Ester HCl ejwayelekile kanye ne-4-Nitro-L-Phenylalanine monohydrate ejwayelekile ku-50-ml ye-volumetric flask, ncibilika futhi uhlambulule kuvolumu nge-methanol bese uxuba.
Ukulungiselela Ukuhlolwa:
Kala ngokunembile futhi udlulise cishe isampula engu-25 mg kufulaski wevolumu engu-50-ml, ncibilikisa bese unciphisa kuvolumu nge-methanol bese uxuba.
Amapharamitha wezinsimbi:
Insimbi: I-HPLC ifakwe umjovo, Iphampu, umtshina we-UV kanye neRekhoda
Ikholomu: XTerra RP-18 (4.6mm x 150mm) 5 μ noma okulinganayo
Izinga lokugeleza: 1.0 ml/ngomzuzu
Ubude begagasi: 265nm
Umthamo womjovo: 20 μL
Izinga lokushisa lekholamu yekholomu: 25℃
Isikhathi sokugijima: imizuzu engama-45
Uhlelo lweGradient
Isikhathi (imizuzu) Isigaba Seselula-A % Isigaba Seselula-B %
0.01 90 10
15 60 40
35 40 60
40 35 65
41 90 10
45 90 10
Inqubo:
Jova izixazululo njengoba kushiwo ngokulandelana okulandelayo.Rekhoda ama-chromatogram futhi uqinisekise imibandela:
Ukulungiselela Okungenalutho 1 Akukho ukuphakama okuphazamisayo okufanele kubonwe ngesikhathi sokugcinwa kwe-L-4-Nitrophenylalanine Methyl Ester HCl.
Ukulungiswa kokufaneleka kwesistimu 1 Isici somsila se-L-4-Nitrophenylalanine Methyl Ester HCl ukuphakama akufanele kube ngaphansi kuka-2.0
Ukulungiselela ukuhlolwa 1 Sebenzisa i-chromatogram yokuhlola ukuthola ubumsulwa be-chromatographic nokungcola.
Izikhathi Zokugcina Ezihlobene:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (isikhathi sokugcina cishe imizuzu eyi-11.4)
4-Nitro-L-Phenylalanine Mayelana ne-0.4
I-Toluene Mayelana ne-2.3
Izibalo:
Ku-chromatogram etholwe ekulungiseleleni ukuhlolwa, ngemva kokulungiswa okungenalutho, ungayinaki inani eliphakeme ngenxa ye-toluene.Bika ukuhlanzeka kwe-chromatographic, okuqukethwe kwe-4-Nitro-L-Phenylalanine kanye nokungcola okuphelele kusampula ngendlela yokujwayela kwendawo.