I-L-Cysteine ​​CAS 52-90-4 (H-Cys-OH) Assay 98.5~101.0% (Titration) Factory High Quality

I-L-Cysteine ​​iqukethe okungengaphansi kuka-98.5% futhi hhayi ngaphezu kuka-101.0% we-L-Cysteine ​​(C3H7NO2S), abalwe ngokomisiwe.
I-L-Cysteine ​​yenzeka njengamakristalu amhlophe noma impushana emhlophe.Inephunga eliyisici kanye nokunambitheka okubabayo.Incibilika ngokukhululeka emanzini, futhi ayincibiliki ku-ethanol (99.5).Incibilika ku-1 mol/L i-hydrochloric acid TS.
Ukuhlonza Thola i-spectrum yokumuncwa kwe-infrared ye-L-Cysteine ​​njengoba kuqondiswe endleleni yediski ye-potassium bromide ngaphansi kwe-Infrared Spectrophotometry <2.25>, futhi uqhathanise i-spectrum ne-Reference Spectrum: yomibili i-spectra ibonisa ukuqina okufanayo kokumuncwa ngezinombolo zamagagasi afanayo.
Ukuzungezisa okubonakalayo <2.49> [a]20D:+8.0 ~+10.0° (2 g kubalwe ngokomisiwe, 1 mol/L hydrochloric acid TS, 25mL, 100 mm).
I-pH <2.54> I-pH yesisombululo esilungiswe ngokuncibilikisa i-1.25 g ye-L-Cysteine ​​​​ku-50 mL yamanzi ingu-4.7 kuya ku-5.7.
Ukuhlanzeka (1) Ukucaca kanye nombala wesixazululo-Diza i-1.0 g ye-L-Cysteine ​​ku-20 mL wamanzi: isixazululo sicacile futhi asinambala.
(2) I-Chloride <1.03>-Chaza u-0.30 g we-L-Cysteine ​​ku-10mL we-nitric acid ehlanjululwe (1 ku-4), engeza u-10mL we-hydrogenperoxide (30), ukushisa imizuzu engu-20 emanzini abilayo okugeza, pholile, bese wengeza amanzi ukwenza 50 ml.Yenza ukuhlolwa usebenzisa lesi sixazululo njengesixazululo sokuhlola.Lungiselela isixazululo sokulawula ngo-0.35 mL ka-0.01 mol/L hydrochloric acid VS (hhayi ngaphezu kuka-0.041%)
(3) I-Sulfate <1.14>-Dilute 0.6 g ye-L-Cysteine ​​ku-30 ​​mL wamanzi kanye no-3 mL we-dilute hydrochloric acid, bese wengeza amanzi ukwenza u-50 mL.Yenza ukuhlolwa usebenzisa lesi sixazululo njengesixazululo sokuhlola.Lungiselela ikhambi lokulawula ngale ndlela elandelayo: Ku-0.35 mL ka-0.005 mol/L sulfuric acid VS engeza u-3 mL we-dilute hydrochloric acid namanzi ukwenza u-50 mL.Lungiselela isixazululo sokuhlola nesixazululo sokulawula nge-4 mL ye-barium chloride TS, ngokulandelana (hhayi ngaphezu kuka-0.028%).
(4) I-Ammonium <1.02>-Yenza ukuhlolwa ngo-0.25 g we-L-Cysteine, usebenzisa i-distillation ngaphansi kwengcindezi encishisiwe.Lungiselela ikhambi lokulawula ngo-5.0 mL we-Standard Ammonium Solution (hhayi ngaphezu kuka-0.02%).
(5) Ama-Heavy Metals <1.07>-Qhubeka ne-1.0g ye-L-Cysteine ​​ngokwe-Method 4, futhi wenze ukuhlolwa.Lungisa ikhambi lokulawula ngo-1.0 mL we-Standard lead Solution (hhayi ngaphezu kuka-10 ppm).
(6)I-Iron <1.10>-Lungisa isixazululo sokuhlola nge-1.0 g ye-L-Cysteine ​​ngokwendlela Yoku-1, futhi wenze ukuhlolwa ngokweNdlela A. Lungiselela isisombululo sokulawula nge-1.0mL ye-Standard Iron Solution (hhayi ngaphezu kuka-10 ppm ).
(7)Izinto Ezihlobene-Chaza u-0.10 g we-L-Cysteine ​​kusixazululo se-N-ethylmaleimide (1 ku-50) ukuze wenze u-10 mL ngqo, shiya imizuzu engu-30, futhi usebenzise lesi sixazululo njengesampula yesixazululo.Ipayipi elingu-1 mL lesixazululo sesampula, engeza amanzi ukwenza u-10 mL ngqo, ipayipi elingu-1 mL lalesi sixazululo, engeza amanzi ukwenza u-50 mL ngqo, bese usebenzisa lesi sixazululo njengesixazululo esijwayelekile (1).Ngokwehlukana, ncibilikisa u-0.10 g we-L-cystine ku-0.5mol/L i-hydrochloric acid TS ukwenza u-20 mL ngqo.Ipayipi elingu-1mL lalesi sixazululo, engeza amanzi ukwenza u-100 ml, bese usebenzisa lesi sixazululo njengesixazululo esijwayelekile (2).Yenza ukuhlolwa ngalezi zixazululo njengoba kuqondiswe ngaphansi kwe-Thin-layer Chromatography<2.03>.Faka i-10mL ngayinye yesixazululo sesampula nezisombululo ezijwayelekile (1) kanye (2) epuletini lejeli yesilika yechromatography yongqimba oluncane.Yakha ipuleti ngengxube ye-1-butanol, amanzi, ne-acetic acid (100) (3:1:1) ukuya ebangeni elingamasentimitha ayi-10, bese womisa ipuleti imizuzu engama-30 ku-809C.Fafaza ipuleti ngokulinganayo ngesixazululo. i-ninhydrin engxubeni ye-methanol ne-acetic acid (100) (97:3) (1 ku-100), bese ishisa ku-80℃ imizuzu eyi-10: indawo etholwe kusixazululo sesampula esihambisana nendawo etholwe esixazululweni esijwayelekile. (2) alinamandla kakhulu kunendawo evela kusixazululo esijwayelekile (2).Futhi, amachashaza ngaphandle kwendawo eyinhloko kanye namachashaza ashiwo ngenhla esixazululweni sesampula awashubi kakhulu kunesixazululo esijwayelekile (1).
Ukulahlekelwa Ekumisweni <2.41> Kungabi ngaphezu kuka-0.50% (1 g, ku-vacuum, i-phosphorus (V) oxide, amahora angu-3).
Insalela Ekushiseni <2.44> Ayidluli ku-0.1%(1 g).
I-Assay Kala ngokunembile mayelana ne-0.2 g ye-L-Cysteine, beka i-itin kufulaski omisiwe, bese uncibilika ku-20 mL wamanzi.Chaza u-4 g we-potassium iodide kulesi sixazululo, ngokushesha ubeke emanzini abandayo eqhwa, engeza u-5 mL we-dilute hydrochloric acid kanye ncamashi no-25 mL we-0.05 mol/L iodine VS, shiya endaweni emnyama imizuzu engu-20, bese u-titrate<2.50 >inani eleqile le-iodine ene-0.1 mol/L ye-sodium thiosulfate VS (inkomba: isitashi TS).Yenza isinqumo esingenalutho usebenzisa indlela efanayo.
I-mL ngayinye ka-0.05 mol/L iodine VS＝12.12 mg ye-C3H7NO2S
Iziqukathi kanye Iziqukathi Storage-Tight iziqukathi.