I-L-Cysteine ​​Hydrochloride Monohydrate CAS 7048-04-6 Assay 98.5~101.0% (Titration on Dried Basis) Factory

I-L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) AJI97 Indlela Yokuhlola
Ukuhlonza: Qhathanisa i-spectrum yokumuncwa kwe-infrared yesampula naleyo yezinga lendlela ye-potassium bromide disc.
Ukuzungezisa Okuthile [α]D20: Isampula eyomile, C=8, 1mol/L HCl
Isimo Sesixazululo (Ukudlulisa): 1.0g ku-10ml we-H2O, i-spectrophotometer, 430nm, 10mm ubukhulu beseli.
I-Chloride (Cl): 350mg, B-2
I-Ammonium (NH4): A-1
I-Sulfate (SO4): 1.2g, (1), ref: 0.50ml ka-0.005mol/L H2SO4
I-ayoni (Fe): 1.5g, ref: 1.5ml ye-Iron Std.(0.01mg/ml)
Izinsimbi Ezisindayo (Pb): 2.0g, (1), pH=7, ref: 2.0ml ye-Pb Std.(0.01mg/ml)
I-Arsenic (As2O3): 2.0g, (1), ref: 2.0ml ye-As2O3 Std.
Amanye ama-Amino Acids: Isampula Yokuhlola: 50μg, B-6-a, ukulawula;L-Cys·HCl·H2O 0.25μg
Ukulahlekelwa Ekumisweni: Ku-vacuum, i-P2O5, ekamelweni lokushisa amahora angu-20.
Izinsalela Ekushiseni (I-Sulfated): Ukuhlolwa kwe-AJI 13
I-Assay: Isampula eyomisiwe, 250mg, 0.05mol/L I2 1ml=15.762mg C3H7NO2S·HCl
Ukuhlolwa kwe-pH: 1.0g ku-100ml we-H2O

I-L-Cysteine ​​Hydrochloride Monohydrate (CAS: 7048-04-6) Indlela Yokuhlola ye-USP35
INCAZELO
I-Cysteine ​​Hydrochloride iyi-L-Cysteine ​​Hydrochloride Monohydrate futhi iqukethe i-NLT 98.5% ne-NMT 101.5% ye-L-Cysteine ​​Hydrochloride (C3H7NO2S·HCl), ebalwa ngokomisiwe.
UKUKHOMBA
A. UKUMUMVA OKUTHI INFRARED <197K>
ISIVIVINYO
INQUBO
Isampula: 250 mg we-Cysteine ​​Hydrochloride
Akunalutho: Qhubeka njengoba kuqondiswe Ekuhlaziyeni ngaphandle Kwesampula.
Isistimu ye-Titrimetric
(Bheka i-Titrimetry <541>)
Imodi: I-titration eyinsalela
I-Titrant: 0.1 N iodine VS
I-back titrant: 0.1 N sodium thiosulfate VS
Ukutholwa kwendawo yokugcina: Okubonakalayo
Ukuhlaziya: Dlulisela Isampula kuflask ye-iodine.Faka u-20.0 mL wamanzi no-4 g we-potassium iodide, uhlanganise.Pholisa isixazululo endaweni yokugezela yeqhwa, bese wengeza u-5 mL we-3 N hydrochloric acid kanye no-25.0 mL we-0.1 N iodine VS.Faka isivalo, futhi uvumele ukuma ebumnyameni imizuzu engu-20.Titrate iodine eyeqile nge-Back titrant.Engeza u-3 mL wenkanyezi ch TS njengoba kusondela isiphetho.Ngokuvumelana nesinqumo esingenalutho.Bala iphesenti le-cysteine ​​​​hydrochloride (C3H7NO2S·HCl) Kusampula ethathiwe:
Umphumela = {[(VB − VS) × N × F]/W} × 100
I-VB = Ivolumu ye-titrant yangemuva edliwe yi-Blank (mL)
VS = Ivolumu ye-titrant yangemuva edliwe yiSampuli (mL)
N = ukujwayelekile kwangempela kwe-Back titrant (mEq/mL)
F= i-equivalency factor, 157.6 mg/mEq
W= Isisindo sesampula (mg)
Indlela yokwamukela: 98.5%~101.5% ngokomisiwe
UKUNGCOLA
ISAZISO EKUSHIYENI <281>: NMT 0.4%
I-CHLORIDE NE-SULFATE, i-Sulfate <221>
Isixazululo esijwayelekile: 0.10 mL ka-0.020 N sulfuric acid
Isampula: 0.33 g ye-Cysteine ​​Hydrochloride
Indlela yokwamukela: NMT 0.03%
I-IRON <241>: NMT 30ppm
IZINSIMBI EZINZIMA, Indlela I <231>: NMT 15ppm
AMACOMPOUNDS AMAQINISO
Isixazululo se-N-Ethylmaleimide: 40 mg/mL ye-N-ethylmaleimide otshwaleni
Isixazululo esijwayelekile sesitoko: Chaza u-20 mg we-USP L-Cysteine ​​Hydrochloride RS ku-10.0 mL wamanzi.Engeza u-10.0 mL wesisombululo se-N-Ethylmaleimide, bese uxuba.Vumela isixazululo sime imizuzu emi-5 ngaphambi kokusebenzisa.
Isixazululo esijwayelekile: 0.05 mg/mL kusuka kusixazululo sesitoko Esijwayelekile emanzini.[QAPHELA-Lesi sixazululo sinokugxila okulingana no-0.5% wesixazululo Sesampuli.]
Isixazululo sokufaneleka kwesistimu: Dlulisa u-10 mg we-USP L-Tyrosine RS kanye no-10 mL wesisombululo sesitoko Esijwayelekile ku-25-mL ye-volumetric flask.Nciphisa ngamanzi ngevolumu.
Isixazululo sesitoko esiyisampula: Dlulisa u-0.2 g we-Cysteine ​​Hydrochloride ku-10-mL ye-volumetric flask, ncibilikisa, futhi hlambulula ngamanzi ukuya kuvolumu.
Isixazululo esiyisampula: Ku-5.0 mL wesixazululo sesitoko esiyisampula engeza u-5.0 mL wesixazululo se-N-Ethylmaleimide, bese uxuba.Vumela isixazululo sime imizuzu emi-5 ngaphambi kokusebenzisa.
Isistimu ye-Chromatographic (Bona i-Chromatography <621>, i-Thin-Layer Chromatography.)
Imodi: TLC
I-Adsorbent: 0.25-mm ungqimba lwengxube yejeli yesilica yechromatographic
Ivolumu yohlelo lokusebenza: 5μL
Ukuthuthukisa uhlelo lokuncibilikisa: I-Butyl alcohol, i-glacial acetic acid, namanzi (3:1:1)
I-reagent yokufutha: 2 mg/mL we-ninhydrin kwingxube ye-butyl alcohol ne-2N acetic acid (95:5)
Ukufaneleka kwesistimu
Izidingo zokufaneleka: Ichromatogram yesisombululo sokufaneleka Kwesistimu ibonisa izindawo ezimbili ezihlukene ngokusobala.
Ukuhlaziya
Amasampula: Isixazululo esijwayelekile, isixazululo sokufaneleka kwesistimu, nesixazululo sesampula.
Ngemva kokumisa ipuleti ngomoya, fafaza nge-Spray reagent, bese ushisa phakathi kuka-100° no-105° imizuzu engu-15.Hlola ipuleti ngaphansi kokukhanya okumhlophe.
Imibandela yokwamukela: Noma iyiphi indawo yesibili yesisombululo Sesampuli ayinkulu noma iqine kakhulu kunendawo eyinhloko yesisombululo Esivamile.
Ukungcola komuntu ngamunye: NMT 0.5%
Ukungcola okuphelele: NMT 2.0%
IZIVIVINYO EZITHILE
UKUZUNGUZA KOMBONISO, Ukuzungezisa Okuqondile <781S>
Isixazululo sesampula: 80 mg/mL ku-6 N hydrochloric acid
Indlela yokwamukela: +5.7° ukuya ku-+6.8°
I-LASSON DRYING <731>: Yomisa isampula ekamelweni lokushisa ngengcindezi engeqile ku-5 mm ye-mer cury amahora angu-24: ilahlekelwa ngu-8.0%~12.0% wesisindo sayo.
IZIDINGO EZENGEZIWE
UKUPAKASHA KANYE NESIGCINI: Londoloza ezitsheni ezivalwe kahle.
I-USP REFERENCE AMAZINGA <11>
I-USP L-Cysteine ​​Hydrochloride RS
I-USP L-Tyrosine RS