I-L-Glutamine CAS 56-85-9 (H-Gln-OH) Isivivinyo 99.0~101.0% Ifektri Yekhwalithi Ephezulu

I-Pharmacopoeia yaseJapaneAmazinga Okubhekisela
I-L-Glutamine [56-85-9]
I-L-Glutamine, uma yomisiwe, iqukethe okungaphansi kuka-99.0% futhi hhayi ngaphezu kuka-101.0% we-L-Glutamine (C5H10N2O3).
I-L-Glutamine yenzeka imhlophe, icwecwe noma impushana ecwebezelayo.Inokunambitheka kwesici esincane
Incibilika ngokukhululekile ku-formic acid, inyibilika emanzini, futhi ayincibiliki ku-ethanol (99.5)
Ukuhlonza Thola i-spectrum yokumuncwa kwe-infrared ye-L-Glutamine njengoba kuqondiswe endleleni yediski ye-potassium bromide ngaphansi kwe-Infrared Spectrophotometry <2.25>, bese uqhathanisa i-spectrum ne-Reference Spectrum: yomibili i-spectra ibonisa ukuqina okufanayo kokumuncwa ngezinombolo zamagagasi afanayo.
I-Optical Rotation <2.49> [a]20D:+6.3° to +7.3° Kala ngokunembile cishe u-2 g we-L-Glutamine, omisiwe ngaphambilini, engeza u-45mL wamanzi, afudumele ukuya ku-40℃ukuze ancibilike, futhi ngemva kokupholisa, engeza amanzi ukuze wenze. ncamashi 50 ml.Nquma ukujikeleza kwe-optical kwalesi sixazululo kuseli engu-100-mm, phakathi nemizuzu engama-60.
I-pH <2.54> I-pH yesisombululo esilungiswe ngokuncibilikisa u-1.0 g we-L-Glutamine ku-50 mL wamanzi iphakathi kuka-4.5 no-6.0.
Ubumsulwa
(1) Ukucaca nombala wesixazululo—Isixazululo esitholwe ngokuncibilikisa u-0.5 g we-L-Glutamine ku-20 mL wamanzi ucacile futhi awunambala.
(2) I-Chloride <1.03> —Yenza ukuhlolwa nge-0.5 g ye-L-Glutamine.Lungiselela isixazululo sokulawula ngo-0.30 mL ka-0.01 mol/L we-hydrochloric acid VS (hhayi ngaphezu kuka-0.021%).
(3) I-Sulfate <1.14>—Yenza ukuhlolwa nge-0.6 g ye-L-Glutamine.Lungiselela ikhambi lokulawula ngo-0.35 mL ka-0.005 mol/L sulfuric acid VS (hhayi ngaphezu kuka-0.028z).
(4) I-Ammonium <1.02>—Yenza ukuhlola nge-0.10 g ye-L-Glutamine, usebenzisa i-distillation ngaphansi kwengcindezi encishisiwe.Lungiselela ikhambi lokulawula ngo-10.0 mL we-Standard Ammonium Solution.Izinga lokushisa lokugeza lamanzi ngu-45 ℃ (hhayi ngaphezu kuka-0.1%)
(5) Izinsimbi Ezisindayo <1.07>—Qhubeka ne-1.0 g ye-L-Glutamine ngokweNdlela 1, futhi wenze ukuhlolwa.Lungiselela isixazululo sokulawula ngo-1.0 mL Wesixazululo Esijwayelekile Sokuhola (hhayi ngaphezu kuka-10 ppm).
(6) I-Iron <1.10>—Lungiselela isixazululo sokuhlola nge-1.0 g ye-L-Glutamine ngokweNdlela 1, futhi wenze ukuhlolwa ngokweNdlela A. Lungiselela isisombululo sokulawula nge-1.0 mL ye-Standard Iron Solution (hhayi ngaphezu kuka-10 ppm)
(7) Izinto Ezihlobene—Chaza u-0.10 g we-L-Glutaminein 10 mL wamanzi, futhi usebenzise lesi sixazululo njengesixazululo sesampula.Ipayipi elingu-1 mL lesixazululo sesampula, engeza amanzi ukwenza u-10 mL ngqo.Ipayipi elingu-1 mL lalesi sixazululo, engeza amanzi ukwenza u-50 mL ncamashi, bese usebenzisa lesi sixazululo njengesixazululo esijwayelekile.Yenza ukuhlolwa ngalezi zixazululo njengoba kuqondiswe ngaphansi kwe-Thin-layer Chromatography <2.03>.Faka i-5mL ngayinye yesixazululo sesampula nesisombululo esijwayelekile epuletini le-silicagel le-chromatography yongqimba oluncane.Bese uthuthukisa ngengxube ye-1-butanol, amanzi ne-acetic acid (100) (3:1:1) iye ebangeni elingama-cm ayi-10, bese womisa ipuleti ku-80℃ imizuzu engama-30.Fafaza ngokulinganayo isixazululo se-ninhydrin engxubeni ye-methanol ne-acetic acid (100) (97:3) (1 ku-100) epuletini, bese ushisa ku-80℃ imizuzu eyi-10: indawo ngaphandle kwendawo eyinhloko etholwe ne Isixazululo sesampula asinamandla kakhulu kunendawo etholwe ngesixazululo esijwayelekile.
Ukulahlekelwa Ekumisweni <2.41> Hhayi ngaphezulu kuka-0.3% (1 g, 105℃, amahora angu-3)
Insalela Ekushiseni <2.44> Ayidluli ku-0.10%(1 g).
I-Assay Kala ngokunembile cishe u-0.15 g we-L-Glutamine, omisiwe ngaphambilini, ncibilika ku-3 mL we-formic acid, engeza u-50 mL we-acetic acid (100), kanye ne-titrate <2.50> ngo-0.1 mol/L perchloric acid VS (potentiometric titration).Yenza isinqumo esingenalutho ngendlela efanayo, futhi wenze noma yikuphi ukulungisa okudingekayo.
I-mL ngayinye ka-0.1 mol/L perchloric acid VS＝14.61 mg ye-C5H10N2O3
Iziqukathi Neziqukathi Zokugcina—Iziqukathi eziqinile.

I-USP Reference Standards <11>-USP Glutamine RS.
Ukuhlonza, I-Infrared Absorption <197K>.
Ukuzungezisa Okuqondile <781S>: phakathi kuka-+6.3° no-+7.3°, kunqunywa ku-20℃
Isixazululo Sokuhlola- Eflaskini efanelekile, lungisa kahle ikhambi elingaba ngu-40mg nge-ml ngayinye emanzini cishe ku-40℃.Pholisa futhi uhlambulule ngamanzi ngevolumu ngaphambi kokusetshenziswa.
Ukulahlekelwa Ekumisweni <731>-Yomisa ku-105 ℃ amahora angu-3;ayilahlekelwa ngaphezu kuka-0.20% wesisindo sayo.
Izinsalela Ekuthutheni <281>Hhayi ngaphezu kuka-0.30%
I-Chloride <221>-Ingxenye engu-0.7-g ayibonisi i-chloride engaphezu kwe-0.50ml ka-0.020 N hydrochloric acid: akutholakali ngaphezu kuka-0.05%.
I-sulfate <221>-Ingxenye ye-0.8-g ayibonisi i-sulfate engaphezu kwe-0.25ml ye-0.020 N i-sulfuric acid: hhayi ngaphezu kwe-0.03% etholakalayo.
I-Chromatographic Purity
I-Adsorbent: 0.25-mm ungqimba lwengxube yejeli yesilica yechromatographic.
Isixazululo sokuhlola: 10mg nge-ml ngayinye, emanzini
Isixazululo Esijwayelekile-Lungiselela isixazululo se-USP Glutamine RS emanzini esinengxube eyaziwayo engaba ngu-0.05mg nge-ml.
Ivolumu yohlelo lokusebenza: 5μL
Ukuthuthukisa Uhlelo Lokuncibilikisa: ingxube ye-butyl alcohol, amanzi, ne-glacial acetic acid (3:1:1).
Futha i-Reagent-Dissolve 0.2g ye-ninhydrin ku-100ml wengxube ye-butyl alcohol kanye no-2 N acetic acid (95:5)
Inqubo-Qhubeka njengoba kuqondiswe ku-chromatography yongqimba oluncane ngaphansi kwe-Chromatography <621>, bese womisa ipuleti kokuthi 80℃ imizuzu engu-30.Fafaza ipuleti nge-reagent yokufutha, shisa ku-80 ℃ imizuzu eyi-10, bese uhlola ngaphansi kokukhanya okumhlophe: ayikho indawo yesibili ku-chromatogram etholwe kusixazululo sokuhlola inkulu noma iqine kakhulu kunendawo eyinhloko ku-chromatogram etholakala kusixazululo se-standrad. (0.5%)
Ukungcola Okuguquguqukayo Kwezinto Eziphilayo, Indlela I <467>: Hlangana nezidingo.
I-Assy-Dlulisela mayelana ne-150mg ye-Glutamine, enesisindo esinembile, ku-flask engu-125ml, i-disolbe ku-3ml ye-formic acid kanye ne-50ml ye-glacial acetic acid, ne-titrate nge-0.1 N perchloric acid VS enquma i-endpoint potentiometrically.Yenza isinqumo esingenalutho, futhi wenze noma yikuphi ukulungisa okudingekayo (bona i-Titrimetry<541>).I-ml ngayinye ka-0.1 N perchloric acid ilingana no-14.615 mg we-C5H10N2O3