I-L-Leucine Methyl Ester Hydrochloride CAS 7517-19-3 (H-Leu-OMe·HCl) Assay >99.0% Factory

1. Ukubukeka
Thatha inani elifanele lalo mkhiqizo, ulibeke ephepheni elimhlophe elibushelelezi futhi usakaze u-5 cm2 no-5 mm ubukhulu.Kubheke endaweni ekhanyayo.
2. Ubumsulwa (HPLC)
2.1 Ithuluzi
Ukusebenza okuphezulu kwe-chromatography ye-liquid, umtshina we-PDA.
Ibhalansi yokuhlaziya ye-elekthronikhi
2.2 Ama-reagents
I-Acetonitrile (ibanga le-chromatographic), i-TFA(ibanga le-chromatographic)
2.3 Izimo zeChromatographic
2.3.1 Ikholomu: YMC-ODS-AM, 5 μl, 150 x 4.6 mm
2.3.2
Ubude begagasi bokutholwa: INC220nm
Izinga lokugeleza: 1.0mL/min
Ivolumu yomjovo: 10 μL (ngokwereferensi)
I-Diluent: i-acetonitrile
Isikhathi sokugijima: imizuzu engu-20
2.4 Ukulungiselela isigaba sikamakhalekhukhwini
Isigaba seselula A (0.1% samanzi e-trifluoroacetic acid): amunca kahle i-2.0ml TFA, Nciphisa ku-2000ml ngamanzi, hlanganisa kanye ne-degas;
Isigaba seselula B (0.1% trifluoroacetic acid acetonitrile ): imunca ngokunembile i-2.0ml TFA, Nciphisa ku-2000ml nge-acetonitrile, mix and degas;
2.5 Amapharamitha wezinsimbi
Isikhathi (amaminithi) A% B%
0.00 90 10
12.00 10 90
15.00 10 90
15.01 90 10
20.00 90 10
2.6 Ukulungiswa kwesixazululo sesampula
Kala bese uncibilikisa isampula engu-0.1g nge-acetonitrile, nciphisa ibe ngu-100ml, xukuza kahle ukuze uyisebenzise, ​​noma ukugxila okufanayo.Lungiselela amasampula amabili ngokuhambisana.
2.7 Ukunqunywa kwesampula
Hlaziya isampula ngokuya ngenqubo yamasampula elandelayo:
Umjovo ongaphezu ko-1 wesixazululo esingenalutho
Isampula yenaliti eyisampula yesixazululo 1#
1 isampula yenaliti yesixazululo 2#
2.8 Ukubalwa kwemiphumela
2.8.1 Indlela yokujwayela yendawo ephakemeyo yasetshenziswa ukubala ubumsulwa be-HPLC ngokudonsa indawo engenalutho.
2.8.2 Isilinganiso sokuchezuka kwesihlobo sokuhlanzeka kwezinalithi ezimbili akumele sibe ngaphezu kuka-1%
2.8.3 Uma imiphumela yayo yomibili imijovo ihlangabezana nemibandela yokwamukelwa, ukuhlanzeka okumaphakathi kuthathwa njengomphumela wokugcina.
3, Indawo encibilikayo -- RY-1 ithuluzi lokuncibilika
4. Ukulahlekelwa endleleni yokuhlola yokomisa
4.1 Izinsimbi:
Ihhavini yokomisa i-thermostatic kagesi, ibhalansi engu-1/10,000.
4.2 Inqubo:
Ebhodleleni lesisindo esiyisicaba elinesisindo esingaguquki kanye nesembozo somlomo womhlabathi omiswe ngokweqile, kala igremu elingu-1 (elinembile ukuya ku-0.0001 igremu) lesampula.Isampula kufanele isatshalaliswe ngokulinganayo ngaphansi kwebhodlela lesisindo ngogqinsi olungekho ngaphezu kuka-10mm, ifakwe kuhhavini wokomisa kagesi we-thermostatic, yome ku-105 ~ 110 ℃ amahora angu-3, ​​bese ingena ekamelweni lokumisa ukuze kuphole. izinga lokushisa lekamelo lokukala.
Ukubala: Ukulahlekelwa ekomisweni %= (M1-M2) ÷M×100
Kuphi: M1: isisindo sesampula nebhodlela lokulinganisa ngaphambi kokoma, amagremu
I-M2: isisindo sesampula kanye nebhodlela lokulinganisa ngemva kokumiswa, ngamagremu
M: Isisindo sesampula, amagremu
5. Ukujikeleza okuqondile
-- Ukuzungeza okuqondile kukalwa ngokuhambisana ne-GB/T613-1988
Ukulungiswa kwesampula: isampula ye-1.0000g yalinganiswa ngokunembile futhi yahanjiswa ebhodleleni le-volumetric engu-50ml ehlanzekile neyomile, amanzi angu-20ml ayengezwa, ibhodlela lalimbozwa futhi linyakaziswa ukuze lincibilike, bese lihlanjululwa esikalini ngamanzi.
Isivivinyo: Lungisa uziro we-gyroscope ngaphambi kokuhlolwa, bese ulayisha ithubhu lokuhlola ngesixazululo sesampula, urekhode i-engeli yokuzungezisa, futhi ubale ukuzungezisa okuthile kwesampula ngefomula elandelayo.
[а]D20 = (r×50) ÷ (L×W)
[а]D20: Ukujikeleza okuqondile kokubona ku-20℃ kwesixazululo sesampula
r: Ukuzungezisa okubonwa ku-20℃ ngesixazululo sesampula
50: Umthamo wesixazululo sesampula esilungisiwe (ml)
w: Isisindo sesampula (g)
L: Ubude beshubhu elijikelezayo lokubuka (dm)