L-(-)-Tyrosine CAS 60-18-4 (H-Tyr-OH) Isivivinyo 98.5~101.5% Ifektri Yekhwalithi Ephezulu

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) AJI 97 Indlela Yokuhlola
Ukuhlonza: Qhathanisa i-spectrum yokumuncwa kwe-infrared yesampula naleyo yezinga lendlela ye-potassium bromide disc.
Ukuzungezisa Okuthile [α]20/D: Isampula Eyomisiwe, C=5, 1mol/L HCl
Isimo Sesixazululo (Ukudlulisa): 0.5g ku-10ml we-1mol/L HCl, i-spectrophotometer, 430nm, 10mm ubukhulu beseli.
I-Chloride (Cl): 0.7g, A-1, ref: 0.40ml ka-0.01mol/L HCl
I-Ammonium (NH4): B-2
I-Sulfate (SO4): 1.2g, (1), ref: 0.50ml ka-0.005mol/L H2SO4
Insimbi (Fe): 1.5g, (2), ref: 1.5ml ye-Iron Std.(0.01mg/ml)
Izinsimbi Ezisindayo (Pb): 2.0g, (2), ref: 2.0ml ye-Pb Std.(0.01mg/ml)
I-Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ye-As2O3 Std.
Amanye Ama-Amino Acid: Dilute ammonia R2 Isampula Yokuhlola: 50μg, F-6-a, control: L-Tyr 0.25μg
Ukulahlekelwa Ekumisweni: ku-105 ℃ amahora angu-3.
Izinsalela Ekushiseni (I-Sulfated): Ukuhlolwa kwe-AJI 13
I-Assay: Isampula esomisiwe, 180mg, (1), 6ml ye-formic acid, 50ml ye-glacial acetic acid, 0.1mol/L HCLO4 1ml=18.119mg C9H11NO3
Ukuhlolwa kwe-pH: 0.02g kusixazululo samanzi esigcwele esingu-100ml

L-(-)-Tyrosine (H-Tyr-OH) (CAS: 60-18-4) Indlela Yokuhlola ye-USP35
INCAZELO
I-Tyrosine iqukethe i-NLT 98.5% kanye ne-NMT 101.5% ye-L-Tyrosine (C9H11NO3), ibalwe ngokomisiwe.
UKUKHOMBA
A. UKUMUMVA OKUTHI INFRARED <197K>
ISIVIVINYO
INQUBO
Isampula: 180 mg yeTyrosine
Okungenalutho: Hlanganisa u-6 mL we-formic acid no-50 mL we-glacial acetic acid.
Isistimu ye-Titrimetric
(Bheka i-Titrimetry <541>)
Imodi: I-titration eqondile
I-Titrant: 0.1 N i-perchloric acid VS
Ukutholwa kwendawo yokugcina: I-Potentiometric
Ukuhlaziya: Chaza Isampula ku-6 mL we-formic acid no-50mL we-glacial acetic acid, kanye ne-titrate ne-Titrant.Yenza ukuzimisela Okungenalutho.
Bala iphesenti le-tyrosine (C9H11NO3) Kusampula ethathiwe:
Umphumela = {[(VS-VB) x N x F]/W} x100
VS= ivolumu ye-Titrant esetshenziswe Isampula (mL)
I-VB= ivolumu ye-Titrant edliwe yi-Blank (mL)
N= ukujwayelekile kwangempela kwe-Titrant (mEq/mL)
F= i-equivalency factor, 181.2 mg/mEq
W= Isisindo sesampula (mg)
Indlela yokwamukela: 98.5%~101.5% ngokomisiwe
UKUNGCOLA
UKUSHISA OKUSELELE <281>: NMT 0.4%
I-CHLORIDE NE-SULFATE, I-Chloride <221>
Isixazululo esijwayelekile: 0.20mL ka-0.020 N hydrochloric acid
Isampula: 0.35 g ye-Tyrosine.[QAPHELA-Uma kunesidingo, ncibilikisa Isampula ngokushisisa eduze nokubilisa bese wengeza u-1 mL we-nitric acid.]
Indlela yokwamukela: NMT 0.04%
I-CHLORIDE NE-SULFATE, i-Sulfate <221>
Isixazululo esijwayelekile: 0.50mL ka-0.020 N sulfuric acid
Isampula: 1.2 g ye-Tyrosine.[QAPHELA-Uma kudingeka, ncibilikisa Isampula ngokungeza u-6 mL we-hydrochloric acid ehlanjululwe.]
Indlela yokwamukela: NMT 0.04%
I-IRON <241>: NMT 30ppm [QAPHELA-Uma kudingeka, sebenzisa u-2 mL we-hydrochloric acid ukuze uncibilikise Isampuli.]
IZINSIMBI EZINZIMA, Indlela I <231>: NMT 15ppm
AMACOMPOUNDS AMAQINISO
Isixazululo se-ammonia esihlanjululwe: Hlanganisa u-16 mL we-ammonia egxilile ngamanzi ku-100 mL.
Isixazululo sokufaneleka kwesistimu: Chaza u-10 mg we-USP L-Tyrosine RS kanye no-10 mg we-USP L-Phenylalanine RS ku-1 mL yesisombululo se-ammonia eDiluted, bese uhlanza ngamanzi abe ngu-25.0 mL.
Isixazululo esijwayelekile: Ku-flask efanelekile, ncibilikisa inani le-USP L-Tyrosine RS ku-1 mL yesixazululo se-ammonia Ehlanjululiwe.Nciphisa ngamanzi ngevolumu ukuze uthole isisombululo esingu-0.05mg/mL.[QAPHELA-Lesi sixazululo sinokugxila okulingana no-0.5% wesixazululo Sesampuli.]
Isixazululo esiyisampula: Dlulisa i-0.1 g ye-Tyrosine ku-10-mL ye-volumetric flask, engeza isixazululo esanele se-ammonia e-Diluted ukuze uncibilike, futhi uhlambulule ngamanzi ngevolumu.
Isistimu ye-Chromatographic (Bona i-Chromatography <621>, i-Thin-Layer Chromatography.)
Imodi: TLC
I-Adsorbent: 0.25-mm ungqimba lwengxube yejeli yesilica yechromatographic
Ivolumu yohlelo lokusebenza: 5μL
Ukuthuthukisa uhlelo lokuncibilikisa: I-Isopropyl alcohol ne-ammonium hydroxide (7:3)
I-reagent yokufutha: 2 mg/mL we-ninhydrin kwingxube ye-butyl alcohol ne-2N acetic acid (95:5)
Ukufaneleka kwesistimu
Izidingo zokufaneleka: Ichromatogram yesisombululo sokufaneleka Kwesistimu ibonisa izindawo ezimbili ezihlukene ngokusobala.
Ukuhlaziya
Amasampula: Isixazululo sokufaneleka kwesistimu, isixazululo esijwayelekile, kanye nesixazululo sesampula.
Yomisa ipuleti phakathi kuka-100 ° no-105 ° kuze kube yilapho i-ammonia inyamalala ngokuphelele.Fafaza nge-spray reagent, bese ushisa phakathi kuka-100 ° no-105 ° imizuzu engu-15.Hlola ipuleti ngaphansi kokukhanya okumhlophe.
Imibandela yokwamukela: Noma iyiphi indawo yesibili yesisombululo Sesampuli ayinkulu noma iqine kakhulu kunendawo eyinhloko yesisombululo Esivamile.
Noma yikuphi ukungcola komuntu ngamunye: NMT 0.5%
Ukungcola okuphelele: NMT 2.0%
IZIVIVINYO EZITHILE
UKUZUNGUZA KOMBONISO, Ukuzungezisa Okuqondile <781S>
Isixazululo sesampula: 50 mg/mL ku-1 N hydrochloric acid
Imibandela yokwamukela: -9.8° ukuya -11.2°
I-LASSSON EKOMENI <731>: Yomisa isampula ku-105℃ amahora angu-3: ilahlekelwa yi-NMT ngo-0.3% wesisindo sayo.
IZIDINGO EZENGEZIWE
UKUPAKASHA KANYE NESIGCINI: Londoloza ezitsheni ezivalwe kahle.
I-USP REFERENCE AMAZINGA <11>
USP L-Phenylalanine RS
I-USP L-Tyrosine RS