I-L-Valine CAS 72-18-4 (H-Val-OH) Isivivinyo 98.5~101.0% Ifektri Yekhwalithi Ephezulu

L-Valine (H-Val-OH) (CAS: 72-18-4) AJI 97 Indlela Yokuhlola
Ukuhlonza: Qhathanisa i-spectrum yokumuncwa kwe-infrared yesampula naleyo yezinga lendlela ye-potassium bromide disc.
Ukuzungezisa Okuthile [α]20/D: Isampula Eyomisiwe, C=8, 6mol/L HCl
Isimo Sesixazululo (Ukudluliswa): 0.5g ku-20ml we-H2O, i-spectrophotometer, 430nm, 10mm ubukhulu beseli.
I-Chloride (Cl): 0.7g, A-1, ref: 0.40ml ka-0.01mol/L HCl
I-Ammonium (NH4): B-2
I-Sulfate (SO4): 1.2g, (1), ref: 0.50ml ka-0.005mol/L H2SO4
Insimbi (Fe): 1.5g, (2), ref: 1.5ml ye-Iron Std.(0.01mg/ml)
Izinsimbi Ezisindayo (Pb): 2.0g, (2), ref: 2.0ml ye-Pb Std.(0.01mg/ml)
I-Arsenic (As2O3): 2.0g, (2), ref: 2.0ml ye-As2O3 Std.
Amanye Ama-Amino Acid: Dilute ammonia R2 Isampula Yokuhlola: 50μg, F-6-a, control: L-Tyr 0.25μg
Ukulahlekelwa Ekumisweni: ku-105 ℃ amahora angu-3.
Izinsalela Ekushiseni (I-Sulfated): Ukuhlolwa kwe-AJI 13
I-Assay: Isampula eyomisiwe, 120mg, (1), 3ml ye-formic acid, 50ml ye-glacial acetic acid, 0.1mol/L HCLO4 1ml=11.715mg C5H11NO2
Ukuhlolwa kwe-pH: 1.0g ku-20ml we-H2O

I-L-Valine (H-Val-OH) (CAS: 72-18-4)Indlela Yokuhlola ye-JP16
I-L-Valine, uma yomisiwe, iqukethe okungengaphansi kuka-98.5% we-C5H11NO2.
I-L-Valine ivela njengamakristalu amhlophe noma i-crystalline powder.Ayinaphunga noma inephunga elibi, futhi inokunambitheka okumnandi kancane, okubabayo.
Incibilika ngokukhululeka ku-formic acid, inyibilika emanzini, futhi ayincibiliki ku-ethanol (95).
Incibilika ku-dilute hydrochloric acid.
Ukuhlonza Thola i-spectrum yokumuncwa kwe-infrared ye-L-Valine, omiswe ngaphambilini, njengoba kuqondiswe endleleni yediski ye-potassium bromide ngaphansi kwe-Infrared Spectrophotometry <2.25>, bese uqhathanisa i-spectrum ne-Reference Spectrum: zombili izibukeli zibonisa amandla afanayo okumunca ezinombolweni zamagagasi ezifanayo. .
Ukuzungezisa okubonakalayo <2.49> [a]20D: +26.5°~+29.0° (ngemuva kokumiswa, 2 g, 6 mol/L hydrochloric acid TS, 25 mL, 100 mm).
pH <2.54> Chaza u-0.5 g we-L-Valine ku-20 mL wamanzi: i-pH yalesi sixazululo iphakathi kuka-5.5 no-6.5.
Ukuhlanzeka (1) Ukucaca kanye nombala wesixazululo Chaza u-0.5 g we-L-Valine ku-20 mL wamanzi: isixazululo sicacile futhi asinambala.
(2) I-Chloride <1.03>-Yenza ukuhlola ngo-0.5 g we-L-Valine.Lungiselela ikhambi lokulawula ngo-0.30 mL we-0.01mol/L hydrochloric acid VS (hhayi ngaphezu kuka-0.021%).
(3) I-Sulfate <1.14>-Yenza ukuhlolwa ngo-0.6 g we-L-Valine.Lungiselela ikhambi lokulawula ngo-0.35 mL we-0.005mol/L sulfuric acid VS (hhayi ngaphezu kuka-0.028%).
(4) I-Ammonium <1.02>-Yenza ukuhlolwa ngo-0.25 g we-L-Valine.Lungiselela ikhambi lokulawula ngo-5.0 mL we-Standard Ammonium Solution (hhayi ngaphezu kuka-0.02%).
(5) Izinsimbi ezinzima <1.07>-Qhubeka no-1.0 g we-L-Valine ngokweNdlela 1, futhi wenze ukuhlolwa.Lungiselela isixazululo sokulawula ngo-2.0 mL Wesixazululo Esijwayelekile Sokuhola (hhayi ngaphezu kuka-20 ppm).
(6) I-Arsenic <1.11>-Qhubeka ne-1.0g ye-L-Valine, lungiselela isisombululo sokuhlola ngokweNdlela yesi-2, futhi wenze ukuhlolwa (hhayi ngaphezu kuka-2 ppm).
(7) Izinto ezihlobene-Chaza u-0.10 g we-L-Valine ku-25mL wamanzi, futhi usebenzise lesi sixazululo njengesixazululo sesampula.Ipayipi elingu-1 mL lesixazululo sesampula, bese wengeza amanzi ukwenza u-50 mL ncamashi.Pipe u-5 ml walesi sixazululo, engeza amanzi ukuze wenze ama-mL angu-20, bese usebenzisa lesi sixazululo njengesixazululo esijwayelekile.Yenza ukuhlolwa ngalezi zixazululo njengoba kuqondiswe ngaphansi kwe-Thin-layer Chromatography <2.03>.Faka i-5mL ngayinye yesixazululo sesampula kanye nesisombululo esijwayelekile epuletini lejeli ye-silica yechromatography yongqimba oluncane.Yakha ipuleti ngengxube ye-1-butanol, amanzi ne-acetic acid (100) (3:1:1) iye ebangeni elingamasentimitha ayi-10, bese womisa ipuleti ku-80℃ imizuzu engama-30.Fafaza ngokulinganayo isixazululo se-ninhydrin ku-acetone (1 ku-50) epuletini, bese ushisisa ku-80℃ imizuzu emi-5: amachashaza ngaphandle kwendawo eyinhloko evela kusixazululo sesampula awashubi kakhulu kunendawo evela esixazululweni esijwayelekile.
Ukulahlekelwa ekumisweni <2.41> Hhayi ngaphezulu kuka-0.30% (1 g, 105℃, amahora angu-3).
Insalela ekuthungeleni <2.44> Ayidluli ku-0.1% (1 g).
I-Assay Kala ngokunembile cishe u-0.12 g we-L-Valine, omisiwe ngaphambilini, futhi incibilika ku-3 mL we-formic acid, engeza u-50 mL we-acetic acid (100), kanye ne-titrate <2.50> ngo-0.1 mol/L perchloric acid VS (potentiometric titration) .Yenza isinqumo esingenalutho, futhi wenze noma yikuphi ukulungisa okudingekayo.
I-mL ngayinye ka-0.1 mol/L i-perchloric acid VS＝11.72 mg ye-C5H11NO2
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