Isisetshenziswa: Insimbi ye-GC (Shimadzu GC-2010)
Ikholomu: DB-17 Agilent 30mX0.53mmX1.0μm
Ukushisa kwe-oven yokuqala: 80 ℃
Isikhathi sokuqala 2.0min
Linganisa 15℃/min
Izinga lokushisa likahhavini lokugcina: 250 ℃
Isikhathi sokugcina 20min
I-Carrier gas Nitrogen
Imodi Ukugeleza Okuqhubekayo
Ukugeleza 5.0mL/min
Isilinganiso sokuhlukaniswa 10:1
Izinga lokushisa lomjovo: 250 ℃
Ukushisa komtshina: 300 ℃
Ivolumu yomjovo 1.0μL
Izinyathelo okufanele zithathwe ngaphambi kokuhlaziywa:
1. Ikholomu yesimo ku-240℃ ubuncane bemizuzu engama-30.
2. Geza isipetu bese uhlanza ijector liner ngendlela efanele ukuze ususe ukungcola kokuhlaziya kwangaphambili.
3. Geza, womise futhi ugcwalise i-diluent ezitsheni zokugeza isirinji.
Ukulungiselela i-Diluent:
Lungiselela isixazululo esingu-2% w/v sodium hydroxide emanzini.
Ukulungiselela okujwayelekile:
Kala cishe u-100mg we-(R)-3-hydroxyprrolidine hydrochloride ejwayelekile ebhodleleni, engeza u-1mL we-diluent bese uncibilikisa.
Ukulungiselela ukuhlolwa:
Kala cishe u-100mg wesampula yokuhlola ebhodleleni, engeza u-1mL we-diluent bese uncibilikisa.Lungiselela ngempinda.
Inqubo:
Jova kungenalutho (i-diluent), ukulungiswa okujwayelekile kanye nokulungiselela ukuhlolwa usebenzisa izimo ze-GC ezingaphezulu.Ziba iziqongo ngenxa yokungenalutho.Isikhathi sokugcina sokuphakama ngenxa ye-(R) -3-hydroxyprrolidine singaba ngu-5.0min.
Qaphela:
Bika umphumela njengesilinganiso
Isikhathi sokuthumela: Nov-13-2021